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dc.contributor.authorXi, X.zh_CN
dc.contributor.authorHan, J. H.zh_CN
dc.contributor.authorZhang, J. Z.zh_CN
dc.contributor.author韩家淮zh_CN
dc.date.accessioned2013-12-12T02:24:44Z
dc.date.available2013-12-12T02:24:44Z
dc.date.issued2001zh_CN
dc.identifier.citationJournal of Biological Chemistry,276(44):41029-41034zh_CN
dc.identifier.issn0021-9258zh_CN
dc.identifier.otherISI:000171925600088zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65845
dc.description.abstractActivation of AMP-activated protein kinase (AMPK) has been recently demonstrated to be associated with 5-aminoimidazole-4-carboxa;de ribonucleoside (AICAR)stimulated glucose transport mediated by both GLUT1 and GLUT4 transporters. However, signaling events upstream and downstream of AMPK are unknown. Here we report that 1) p38 mitogen-activated protein kinase (MAPK) and mitogen-activated protein kinase kinase 3 (MKK3) were activated by AICAR in Clone 9 cells, which express only the GLUT1 transporters, and 2) activation of p38 was required for AICAR-stimulated glucose transport since treatment of the cells with p38 inhibitor SB203580 or overexpression of dominant negative p38 mutant inhibited glucose transport. Moreover, we found that overexpression of the constitutively active form of AMPK mutant also resulted in a significant activation of p38, and inhibition of p38 activity by SB203580 did not affect AICAR-stimulated activation of AMPK. These findings demonstrate that AICAR-stimulated activation of p38 is indeed mediated by AMPK, and the p38 MAPK cascade is downstream of AMPK in the signaling pathway of AICAR-stimulated glucose transport in Clone 9 cells.zh_CN
dc.language.isoen_USzh_CN
dc.subjectMAP KINASEzh_CN
dc.subject3T3-L1 ADIPOCYTESzh_CN
dc.subjectSKELETAL-MUSCLEzh_CN
dc.subjectOXIDATIVE-PHOSPHORYLATIONzh_CN
dc.subject5-AMINOIMIDAZOLE-4-CARBOXAMIDE RIBONUCLEOSIDEzh_CN
dc.subjectSELECTIVE ACTIVATIONzh_CN
dc.subjectGLUT4 TRANSLOCATIONzh_CN
dc.subjectSIGNAL-TRANSDUCTIONzh_CN
dc.subjectCLONE-9 CELLSzh_CN
dc.subjectINSULINzh_CN
dc.titleStimulation of glucose transport by AMP-activated protein kinase via activation of p38 mitogen-activated protein kinasezh_CN
dc.typeArticlezh_CN


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