Show simple item record

dc.contributor.authorKim, S. O.zh_CN
dc.contributor.authorHa, S. D.zh_CN
dc.contributor.authorLee, S.zh_CN
dc.contributor.authorStanton, S.zh_CN
dc.contributor.authorBeutler, B.zh_CN
dc.contributor.authorHan, J. H.zh_CN
dc.contributor.author韩家淮zh_CN
dc.date.accessioned2013-12-12T02:24:40Z
dc.date.available2013-12-12T02:24:40Z
dc.date.issued2007zh_CN
dc.identifier.citationBiotechniques,42(4):493-501zh_CN
dc.identifier.issn0736-6205zh_CN
dc.identifier.otherISI:000248949200028zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65758
dc.description.abstractDiploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6-8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as tat-gets for insertional mutagenesis using a specially designed retroviral vector that permits rapid identification of disrupted genes in each cell that bears a phenotype of interest. The utility of combined chemical and insertional mutagenesis is illustrated by the identification of novel host genes that are required for macrophage sensitivity to anthrax lethal factor.zh_CN
dc.language.isoen_USzh_CN
dc.subjectEMBRYONIC STEM-CELLSzh_CN
dc.subjectNECROSIS-FACTOR-ALPHAzh_CN
dc.subjectFUNCTIONAL GENOMICSzh_CN
dc.subjectRNA INTERFERENCEzh_CN
dc.subjectEXPRESSION CLONINGzh_CN
dc.subjectPLASMA-MEMBRANEzh_CN
dc.subjectMURINE GENOMEzh_CN
dc.subjectDEATHzh_CN
dc.subjectDISRUPTIONzh_CN
dc.subjectRESISTANCEzh_CN
dc.titleMutagenesis by retroviral insertion in chemical mutagen-gene rated quasi-haploid mammalian cellszh_CN
dc.typeArticlezh_CN


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record