Mutagenesis by retroviral insertion in chemical mutagen-gene rated quasi-haploid mammalian cells
Kim, S. O.
Ha, S. D.
Han, J. H.
- 生命科学－已发表论文 
Diploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6-8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as tat-gets for insertional mutagenesis using a specially designed retroviral vector that permits rapid identification of disrupted genes in each cell that bears a phenotype of interest. The utility of combined chemical and insertional mutagenesis is illustrated by the identification of novel host genes that are required for macrophage sensitivity to anthrax lethal factor.