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dc.contributor.authorSavaldi-Goldstein, Sigalzh_CN
dc.contributor.authorBaiga, Thomas J.zh_CN
dc.contributor.authorPojer, Florencezh_CN
dc.contributor.authorDabi, Tsegeyezh_CN
dc.contributor.authorButterfield, Cristinazh_CN
dc.contributor.authorParry, Geraintzh_CN
dc.contributor.authorSantner, Aaronzh_CN
dc.contributor.authorDharmasiri, Nihalzh_CN
dc.contributor.authorTao, Yizh_CN
dc.contributor.authorEstelle, Markzh_CN
dc.contributor.authorNoel, Joseph P.zh_CN
dc.contributor.authorChory, Jozh_CN
dc.date.accessioned2013-12-12T02:24:36Z
dc.date.available2013-12-12T02:24:36Z
dc.date.issued2008zh_CN
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America,105(39):15190-15195zh_CN
dc.identifier.issn0027-8424zh_CN
dc.identifier.otherWOS:000261914300064zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65680
dc.description.abstractPlant growth depends on the integration of environmental cues and phytohormone-signaling pathways. During seedling emergence, elongation of the embryonic stem (hypocotyl) serves as a readout for light and hormone-dependent responses. We screened 10,000 chemicals provided exogenously to light-grown seedlings and identified 100 compounds that promote hypocotyl elongation. Notably, one subset of these chemicals shares structural characteristics with the synthetic auxins, 2,4-dichlorophenoxyacetic acid (2,4-D), and 1-naphthaleneacetic acid (1-NAA); however, traditional auxins (e. g., indole-3-acetic acid [IAA], 2,4-D, 1-NAA) have no effect on hypocotyl elongation. We show that the new compounds act as "proauxins'' akin to prodrugs. Our data suggest that these compounds diffuse efficiently to the hypocotyls, where they undergo cleavage at varying rates, releasing functional auxins. To investigate this principle, we applied a masking strategy and designed a pro-2,4-D. Unlike 2,4-D alone, this pro-2,4-D enhanced hypocotyl elongation. We further demonstrated the utility of the proauxins by characterizing auxin responses in light-grown hypocotyls of several auxin receptor mutants. These new compounds thus provide experimental access to a tissue previously inaccessible to exogenous application of auxins. Our studies exemplify the combined power of chemical genetics and biochemical analyses for discovering and refining prohormone analogs with selective activity in specific plant tissues. In addition to the utility of these compounds for addressing questions related to auxin and light-signaling interactions, one can envision using these simple principles to study other plant hormone and small molecule responses in temporally and spatially controlled ways.zh_CN
dc.language.isoen_USzh_CN
dc.subjectchemical geneticszh_CN
dc.subjectlightzh_CN
dc.titleNew auxin analogs with growth-promoting effects in intact plants reveal a chemical strategy to improve hormone deliveryzh_CN
dc.typeArticlezh_CN


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