Kinetics of inhibition of green crab (Scylla serrata) alkaline phosphatase by sodium (2,2 '-bipyridine) oxodiperoxovanadate
Zhou, X. W.
Zhuang, Z. L.
Chen, Q. X.
- 生命科学－已发表论文 
Green crab (Scylla serrata) alkaline phosphatase (EC 22.214.171.124) is a metalloenzyme, which catalyzes the nonspecific hydrolysis of phosphate monoesters. The kinetics of inhibition of the enzyme by sodium (2, 2'-bipyridine) oxodiperoxovanadate, pV(bipy), has been studied. The time course of the hydrolysis of p-nitrophenyl-phosphate catalyzed by the enzyme in the presence of different pV(bipy) concentrations showed that at each pV(bipy) concentration, the rate decreased with increasing time until a straight line was approached, the straight line slopes are the same for all concentrations. The results suggest that the inhibition of the enzyme by pV(bipy) is a slow, reversible reaction with fractional remaining activity. The microscopic rate constants are determined for the reaction of inhibitor with the enzyme.