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dc.contributor.authorChen, Q. X.zh_CN
dc.contributor.authorZhang, R. Q.zh_CN
dc.contributor.authorXue, X. Z.zh_CN
dc.contributor.authorYang, P. Z.zh_CN
dc.contributor.authorChen, S. L.zh_CN
dc.contributor.authorZhou, H. M.zh_CN
dc.contributor.author陈清西zh_CN
dc.date.accessioned2013-12-12T02:24:34Z
dc.date.available2013-12-12T02:24:34Z
dc.date.issued2000-04zh_CN
dc.identifier.citationBiochemistry-Moscow, 2000,65(4):452-456zh_CN
dc.identifier.issn0006-2979zh_CN
dc.identifier.otherISI:000087399400008zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65649
dc.description.abstractPrawn (Penaeus penicillatus) acid phosphatase (EC 3.1.3.2) catalyzes the nonspecific hydrolysis of phosphate monoesters. The effects of some pollutants in sea water on the enzyme activity results in the loss of the biological function of the enzyme, which leads to disruption of phosphate metabolism in cells. This paper analyzes the effects of methanol on the activity and conformation of prawn acid phosphatase. The results show that low concentrations of methanol can lead to reversible inactivation. Inhibition of the enzyme by methanol is classified as non-competitive inhibition, and the inhibition constant (K-i) is 8.5%. Conformational changes of the enzyme molecule in methanol solutions of different concentrations were measured using fluorescence emission, differential W-absorption, and circular dichroism spectra. Increased methanol concentrations caused the fluorescence emission intensity of the enzyme to increase. The ultraviolet difference spectra of the enzyme denatured with methanol had two negative peaks, at 222 and 270 nm, and a positive peak at 236 nm, The changes in the fluorescence and ultraviolet difference spectra reflected the changes of the microenvironments of tryptophan and tyrosine residues of the enzyme. The CD spectrum changes of the enzyme show that the secondary structure of the enzyme also changed some. These results suggest that methanol is a non-competitive inhibitor and the conformational integrity of the enzyme is essential for its activity.zh_CN
dc.language.isoen_USzh_CN
dc.subjectCRAB SCYLLA-SERRATAzh_CN
dc.subjectGUANIDINIUM CHLORIDEzh_CN
dc.subjectCREATINE-KINASEzh_CN
dc.subjectALKALINE-PHOSPHATASEzh_CN
dc.subjectINACTIVATIONzh_CN
dc.subjectDENATURATIONzh_CN
dc.subjectKINETICSzh_CN
dc.subjectINHIBITIONzh_CN
dc.subjectSITESzh_CN
dc.subjectRATESzh_CN
dc.titleEffect of methanol on the activity and conformation of acid phosphatase from the prawn Penaeus penicillatuszh_CN
dc.typeArticlezh_CN


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