An essential tryptophan residue of green crab (Syclla serrata) alkaline phosphatase
Zheng, W. Z.
Chen, Q. X.
Zhou, H. M.
- 生命科学－已发表论文 
The, tryptothan residues in green crab (scylla serrata) alkaline phosphatase (EC 126.96.36.199) have been modified by N-bromosuccinimide (NBS). The modification of five tryptophan residues leads to complete loss of enzymatic activity. With the increase of NBS concentration, both the absorption at 278 nm and the fluorescence emission intensity at 335 nm of the modified enzyme decreased markedly indicating the modification of tryptophan residues. Quantitative treatment of the data (Tsou, Sci. Sinica 1962, 11, 1535-1558) shows that among the tryptophan residues modified, one is essential for its catalytic activity. The presence of the substrate markedly protects the modification of tryptophan residues as well as the inactivation, suggesting that the essential tryptophan residue is situated at the active site of this enzyme.