Molecular design of antibrowning agents: antioxidative tyrosinase inhibitors
Chen, Q. X.
- 生命科学－已发表论文 
Dodecyl (C-12) gallate (3,4,5-trihydroxybenzoate) was found to inhibit the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) catalyzed by tyrosinase. This inhibition is a slow and reversible reaction with residual enzyme activity. The inhibition kinetics analyzed by Lineweaver-Burk plots revealed that dodecyl (lauryl) gallate is an uncompetitive inhibitor (K-IS=0.636 mM). Gallic acid, the parent compound of dodecyl gallate, acts as a reducing agent for o-quinone produced by the enzymic oxidation. (C) 2002 Elsevier Science Ltd. All rights reserved.