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dc.contributor.authorYu, Haizh_CN
dc.contributor.authorYuan, Quanzh_CN
dc.contributor.authorGe, Sheng-Xiangzh_CN
dc.contributor.authorWang, Hurng-Yizh_CN
dc.contributor.authorZhang, Ya-Lizh_CN
dc.contributor.authorChen, Qing-Ruizh_CN
dc.contributor.authorZhang, Junzh_CN
dc.contributor.authorChen, Pei-Jerzh_CN
dc.contributor.authorXia, Ning-Shaozh_CN
dc.contributor.author葛胜祥zh_CN
dc.date.accessioned2013-12-12T02:24:22Z
dc.date.available2013-12-12T02:24:22Z
dc.date.issued2010-02-19zh_CN
dc.identifier.citationPlos One, 2010,5(2):zh_CN
dc.identifier.issn1932-6203zh_CN
dc.identifier.otherWOS:000274923700008zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65472
dc.descriptionNational Scientific and Technological Major Project [2008ZX10002-012]; Excellent Youth Foundation of Fujian Province [2009J06020]zh_CN
dc.description.abstractA novel hepatitis B virus (HBV) strain (W29) was isolated from serum samples in the northwest of China. Phylogenetic and distance analyses indicate that this strain is grouped with a series of distinct strains discovered in Vietnam and Laos that have been proposed to be a new genotype I. TreeOrderScan and GroupScan methods were used to study the intergenotype recombination of this special group. Recombination plots and tree maps of W29 and these putative genotype I strains exhibit distinct characteristics that are unexpected in typical genotype C strains of HBV. The amino acids of P gene, S gene, X gene, and C gene of all genotypes (including subtypes) were compared, and eight unique sites were found in genotype I. In vitro and in vivo experiments were also conducted to determine phenotypic characteristics between W29 and other representative strains of different genotypes obtained from China. Secretion of HBsAg in Huh7 cells is uniformly abundant among genotypes A, B, C, and I (W29), but not genotype D. HBeAg secretion is low in genotype I (W29), whose level is close to genotype A and much lower than genotypes B, C, and D. Results from the acute hydrodynamic injection mouse model also exhibit a similar pattern. From an overview of the results, the viral markers of W29 (I1) in Huh7 cells and mice had a more similar level to genotype A than genotype C, although the latter was closer to W29 in distance analysis. All evidence suggests that W29, together with other related strains found in Vietnam and Laos, should be classified into a new genotype.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1371/journal.pone.0009297zh_CN
dc.subjectSURFACE-ANTIGENzh_CN
dc.subjectMOUSE MODELzh_CN
dc.subjectVIRAL-DNAzh_CN
dc.subjectSUBTYPESzh_CN
dc.subjectSTRAINSzh_CN
dc.subjectSUBGENOTYPESzh_CN
dc.subjectRELATEDNESSzh_CN
dc.subjectINFECTIONzh_CN
dc.subjectGENOMESzh_CN
dc.titleMolecular and Phylogenetic Analyses Suggest an Additional Hepatitis B Virus Genotypezh_CN
dc.typeArticlezh_CN


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