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dc.contributor.author王勤zh_CN
dc.date.accessioned2013-12-12T02:24:18Z
dc.date.available2013-12-12T02:24:18Z
dc.date.issued2012-01zh_CN
dc.identifier.citationGlycobiology, 2012,22(1):23-34zh_CN
dc.identifier.issn0959-6658zh_CN
dc.identifier.otherWOS:000297864400005zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65388
dc.descriptionNational Foundation for Fostering Talents of Basic Science [J1030626]; Natural Science Foundation of China [40576066]; Science and Technology Foundation of Fujian Province [2006N0067]; Fujian Province Universityzh_CN
dc.description.abstractThe glycoside hydrolase 18 (GH18) family of chitinases is a multigene family that plays various roles, such as ecdysis, embryonic development, allergic inflammation and so on. Efforts are still needed to reveal their functional diversification in an evolutionary and systematic manner. We collected 85 GH18 genes from eukaryotic representatives. The domain architectures of GH18 proteins were analyzed and several conserved patterns were identified. It was observed that some (11 proteins) GH18 members in Ecdysozoa or fungi possess repeats of catalytic domains and/or chitin-binding domains (ChtBs). The domain repeats are likely to meet requirements for higher efficiency of chitin degradation in chitin-containing species. On the contrary, all vertebrate GH18 proteins contain no more than one catalytic domain or ChtB. The results from homologous analysis, domain architectures, exon arrangements and synteny loci supported two evolutionary paths for the GH18 family. One path experienced gene expansion and contraction several times during evolution, covering most of GH18 members except CHID1 (stabilin-1 interacting partner) and its homologs. Proteins in this path underwent frequent domain gain and loss, as well as domain recombination, that could achieve versatility in function. The other path is comparatively conserved. The CHID1 gene evolved without gene duplication except in Danio rerio. Domain architectures of CHID1 orthologs are all identical. The diverse phylogeny of the GH18 family in arthropod is also presented.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1093/glycob/cwr092zh_CN
dc.subjectACIDIC MAMMALIAN CHITINASEzh_CN
dc.subjectPRAWN PENAEUS-JAPONICUSzh_CN
dc.subjectDROSOPHILA-MELANOGASTERzh_CN
dc.subjectSEQUENCE ALIGNMENTzh_CN
dc.subjectINSECT CHITINASEzh_CN
dc.subjectBINDING DOMAINSzh_CN
dc.subjectBOMBYX-MORIzh_CN
dc.subjectBMCHI-Hzh_CN
dc.subjectPROTEINSzh_CN
dc.subjectGENEzh_CN
dc.titleThe GH18 family of chitinases: Their domain architectures, functions and evolutionszh_CN
dc.typeArticlezh_CN


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