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dc.contributor.authorGao, Yanhuizh_CN
dc.contributor.authorXu, Jiangbozh_CN
dc.contributor.authorXu, Jiaxingzh_CN
dc.contributor.authorHuang, Yaojianzh_CN
dc.contributor.authorShen, Yuemaozh_CN
dc.contributor.authorLiu, ZhaoPingzh_CN
dc.contributor.author沈月毛zh_CN
dc.date.accessioned2013-12-12T02:24:17Z
dc.date.available2013-12-12T02:24:17Z
dc.date.issued2012-01-1zh_CN
dc.identifier.citationJournal of Chromatography B-Analytical Technologie, 2012,8801-5zh_CN
dc.identifier.issn1570-0232zh_CN
dc.identifier.otherWOS:000299197600001zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65365
dc.descriptionNational High Technology Research and Development Program (863) of China [2007AA091503]zh_CN
dc.description.abstractThe purpose of this study was to develop and validate a high-performance liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for analysis of the deacetyl mycoepoxydiene in rat plasma. The analyte and internal standard (IS.), benorilate, were extracted from rat plasma by precipitation protein and separated on a C(18) column using acetonitrile-0.5% formic acid as mobile phase. Detection was performed using a turbo-spray ionization source and mass spectrometric positive multi-reaction-monitoring-mode (+MRM) at an ion voltage of +4800 V. The assay was linear over the concentration range 5-5000 ng/mL with the lowest limit of quantification (LLOQ) of 5 ng/mL. The method also afforded satisfactory results in terms of the sensitivity, specificity, precision (Ultra- and inter-day, RSD <5.8%), accuracy, recovery as well as the stability of the analyte under various conditions. The method was successfully applied to a preclinical pharmacokinetic study in rats after a single intravenous administration of deacetyl mycoepoxydiene 10 mg/kg. (C) 2011 Published by Elsevier B.V.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1016/j.jchromb.2011.06.044zh_CN
dc.subjectDEACETYLMYCOEPOXYDIENEzh_CN
dc.subjectCHROMATOGRAPHYzh_CN
dc.subjectMETABOLITESzh_CN
dc.titleA rapid and sensitive LC-MS/MS assay for the quantitation of deacetyl mycoepoxydiene in rat plasma with application to preclinical pharmacokinetics studieszh_CN
dc.typeArticlezh_CN


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