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dc.contributor.authorZhang, Y.zh_CN
dc.contributor.authorNeo, S. Y.zh_CN
dc.contributor.authorHan, J. H.zh_CN
dc.contributor.authorLin, S. C.zh_CN
dc.contributor.author林圣彩zh_CN
dc.date.accessioned2013-12-12T02:24:13Z
dc.date.available2013-12-12T02:24:13Z
dc.date.issued2000zh_CN
dc.identifier.citationJournal of Biological Chemistry,275(32):25008-25014zh_CN
dc.identifier.issn0021-9258zh_CN
dc.identifier.otherWOS:000088683300103zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65306
dc.description.abstractAxin and Dishevelled are two downstream components of the Wnt signaling pathway. Dishevelled is a positive regulator and is placed genetically between Frizzled and glycogen synthase kinase-3 beta, whereas Axin is a negative regulator that acts downstream of glycogen synthase kinase-3 beta. It is intriguing that they each can activate the c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) when expressed in the cell. We set out to address if Axin and Dishevelled are functionally cooperative, antagonistic, or entirely independent, in terms of the JNK activation event. We found that in contrast to Axin, Dv12 activation of JNK does not require MEKK1, and complex formation between Dv12 and Axin is independent of Axin-MEKK1 binding. Furthermore, Dv12-DIX and Dv12-Delta DEP proteins deficient for JNK activation can attenuate Axin-activated JNK activity by disrupting Axin dimerization. However, Axin-Delta MID, and Axin-Delta T proteins deficient for JNK activation cannot interfere with Dv12-activated JMK activity. These results indicate that unlike the strict requirement of homodimerization for Axin function, Dv12 can activate JNK either as a monomer or homodimer/heterodimer. We suggest that there may be a switch mechanism based on dimerization combinations, that commands cells to activate Wnt signaling or JNK activation, and to turn on specific activators of JNK in response to various environmental cues.zh_CN
dc.language.isoen_USzh_CN
dc.subjectWNT SIGNALING PATHWAYzh_CN
dc.subjectGLYCOGEN-SYNTHASE KINASE-3-BETAzh_CN
dc.subjectBETA-CATENINzh_CN
dc.subjectNEGATIVE REGULATORzh_CN
dc.subjectDOMAINSzh_CN
dc.subjectTRANSCRIPTIONzh_CN
dc.subjectJNKzh_CN
dc.subjectPOLARITYzh_CN
dc.subjectPHOSPHORYLATIONzh_CN
dc.subjectIDENTIFICATIONzh_CN
dc.titleDimerization choices control the ability of axin and dishevelled to activate c-jun N-terminal kinase/stress-activated protein kinasezh_CN
dc.typeArticlezh_CN


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