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dc.contributor.authorChen, Y.zh_CN
dc.contributor.authorNi, H.zh_CN
dc.contributor.authorMa, X. H.zh_CN
dc.contributor.authorHu, S. J.zh_CN
dc.contributor.authorLuan, L. M.zh_CN
dc.contributor.authorRen, G.zh_CN
dc.contributor.authorZhao, Y. C.zh_CN
dc.contributor.authorLi, S. J.zh_CN
dc.contributor.authorDiao, H. L.zh_CN
dc.contributor.authorXu, X.zh_CN
dc.contributor.authorZhao, Z. A.zh_CN
dc.contributor.authorYang, Z. M.zh_CN
dc.contributor.author杨增明zh_CN
dc.date.accessioned2013-12-12T02:24:10Z
dc.date.available2013-12-12T02:24:10Z
dc.date.issued2006zh_CN
dc.identifier.citationJournal of Molecular Endocrinology,37(1):147-161zh_CN
dc.identifier.issn0952-5041zh_CN
dc.identifier.otherISI:000240020600013zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65251
dc.description.abstractAlthough implantation types differ between species, the interaction between blastocyst trophectoderm and apical surface of the uterine epithelium is a common event during the implantation process. In this study, uterine luminal epithelium at implantation and inter-implantation sites was isolated by enzymatic digestion and used for microarray analysis. In a mouse microarray containing 12 345 unigenes, we found 136 genes upregulated more than twofold at the implantation site, while 223 genes were downregulated by at least twofold. Reverse transcription-PCR was used to verify the differential expression of seven upregulated and six downregulated genes chosen randomly from our microarray analysis, and the expression trends were similar. The differential expression patterns of eight upregulated genes were verified by in situ hybridization. Compared with the inter-implantation site on day 5 of pregnancy and the uterus on day 5 of pseudopregnancy, protease, serine, 12 neurotrypsin, endothelin-1, gamma-glutamyl hydrolase, Ras homolog gene family, member U, T-cell immunoglobulin, and mucin domain containing 2, proline-serine-threonine phosphatase-interacting protein 2, 3-monooxgenase/tryptophan 5-monooxgenase activation protein, gamma-polypeptide, and cysteine-rich protein 61 (Cyr61) were upregulated in the luminal epithelium at implantation site on day 5 of pregnancy. These genes may be related to embryo apposition and adhesion during embryo implantation. Cyr61, a gene upregulated at the implantation site, was chosen to examine its expression and regulation during the periimplantation period by in situ hybridization. Cyr61 mRNA was specifically localized in the luminal epithelium surrounding the implanting blastocyst at day 4 midnight and on day 5 of pregnancy, and in the activated uterus, but not expressed in inter-implantation sites and under delayed implantation, suggesting a role for Cyr61 in mediating embryonic-uterine dialog during embryo attachment. Our data could be a valuable source for future study on embryo implantation.zh_CN
dc.language.isoen_USzh_CN
dc.subjectIMMEDIATE-EARLY GENEzh_CN
dc.subjectEMBRYO IMPLANTATIONzh_CN
dc.subjectUTERINE RECEPTIVITYzh_CN
dc.subjectMOLECULAR MARKERSzh_CN
dc.subjectENDOTHELIAL-CELLSzh_CN
dc.subjectCDNA MICROARRAYzh_CN
dc.subject14-3-3 PROTEINSzh_CN
dc.subjectDECIDUAL CELLSzh_CN
dc.subjectGROWTH-FACTORzh_CN
dc.subjectCYR61zh_CN
dc.titleGlobal analysis of differential luminal epithelial gene expression at mouse implantation siteszh_CN
dc.typeArticlezh_CN


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