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dc.contributor.authorHuo, L. J.zh_CN
dc.contributor.authorLiang, C. G.zh_CN
dc.contributor.authorYu, L. Z.zh_CN
dc.contributor.authorZhong, Z. S.zh_CN
dc.contributor.authorYang, Z. M.zh_CN
dc.contributor.authorFan, H. Y.zh_CN
dc.contributor.authorChen, D. Y.zh_CN
dc.contributor.authorSun, Q. Y.zh_CN
dc.contributor.author杨增明zh_CN
dc.date.accessioned2013-12-12T02:24:09Z
dc.date.available2013-12-12T02:24:09Z
dc.date.issued2005zh_CN
dc.identifier.citationReproduction,129(4):403-409zh_CN
dc.identifier.issn1470-1626zh_CN
dc.identifier.otherISI:000228781400003zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65228
dc.description.abstractThe present study investigated the subcellular localization of inducible nitric oxide synthase (iNOS) during mouse oocyte meiotic maturation and fertilization using confocal microscopy, and further studied the roles of iNOS-derived NO in oocyte maturation by using an iNOS-specific inhibitor aminoguanidine (AG) and iNOS antibody microinjection. In germinal vesicle-stage oocytes, iNOS immunoreactivity was mainly localized in the germinal vesicle. Shortly after germinal vesicle breakdown, the iNOS immunoreactivity accumulated around the condensed chromosomes. At metaphase I and metaphase II, with the organization of chromosomes to the equatorial plate, iNOS immunoreactivity was concentrated around the aligned chromosomes, putatively the position of the metaphase spindle. The accumulation of iNOS immunoreactivity could not be detected at anaphase I and anaphase II. However, at telophase I and telophase II, the staining of iNOS was concentrated in the region between the separating chromosomes/chromatids. Furthermore, the staining of iNOS also accumulated in the male and female pronuclei in fertilized eggs. Germinal vesicle breakdown and the first polar body emission of the oocytes were significantly blocked by the iNOS-specific inhibitor AG in a dose-dependent manner. The germinal vesicle breakdown in oocytes injected with NOS antibody was also inhibited. We found that the phosphorylation of mitogen-activated protein kinase in oocytes after germinal vesicle breakdown was inhibited by AG treatment. The control oocytes extruded a normal first polar body, while the AG-treated oocytes exhibited an elongated protrusion or no elongated protrusion. The results of confocal microscopy showed that the AG-treated oocytes were arrested at anaphase I-telophase 1. Our results suggest that the iNOS-derived NO pathway plays important roles in mouse oocyte meiotic maturation, especially in germinal vesicle breakdown and the anaphase-telophase transition.zh_CN
dc.language.isoen_USzh_CN
dc.subjectMEIOTIC MATURATIONzh_CN
dc.subjectFOLLICULAR DEVELOPMENTzh_CN
dc.subjectRAT OVARYzh_CN
dc.subjectIN-VITROzh_CN
dc.subjectEMBRYONIC MITOSISzh_CN
dc.subjectEXPRESSIONzh_CN
dc.subjectOVULATIONzh_CN
dc.subjectLOCALIZATIONzh_CN
dc.subjectINVOLVEMENTzh_CN
dc.subjectRABBITzh_CN
dc.titleInducible nitric oxide synthase-derived nitric oxide regulates germinal vesicle breakdown and first polar body emission in the mouse oocytezh_CN
dc.typeArticlezh_CN


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