Show simple item record

dc.contributor.authorYang, Z. M.zh_CN
dc.contributor.authorDas, S. K.zh_CN
dc.contributor.authorWang, J.zh_CN
dc.contributor.authorSugimoto, Y.zh_CN
dc.contributor.authorIchikawa, A.zh_CN
dc.contributor.authorDey, S. K.zh_CN
dc.contributor.author杨增明zh_CN
dc.date.accessioned2013-12-12T02:24:08Z
dc.date.available2013-12-12T02:24:08Z
dc.date.issued1997zh_CN
dc.identifier.citationBiology of Reproduction,56(2):368-379zh_CN
dc.identifier.issn0006-3363zh_CN
dc.identifier.otherISI:A1997WF23600010zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65195
dc.description.abstractProstaglandins (PGs), especially PGE(2) and PGF(2 alpha), are considered important for blastocyst spacing, implantation, and decidualization in the rodent uterus. However, information regarding uterine sites of PG actions in these processes is lacking. PGE(2) or PGF(2 alpha) interacts with specific G protein-coupled membrane receptors. PGE(2) receptors are classified into four subtypes, EP(1), EP(2), EP(3), and EP(4). While EP(1) is coupled to Ca2+ mobilization, activation of EP(2) and EP(4) triggers stimulation of adenylyl cyclase. In contrast, activation of EP(3) inhibits adenylyl cyclase. PGF(2 alpha) receptor (FP) is coupled to stimulation of phospholipase C-inositol trisphosphate (IP3) pathway and Ca2+ mobilization. This investigation demonstrates that PGE(2) and PGF(2 alpha) receptor genes are expressed in a temporal and cell-specific manner in the periimplantation mouse uterus. In the mouse, the attachment reaction occurs in the evening (2200-2300 h) of Day 4 of pregnancy and is preceded by embryo spacing, uterine edema, and luminal closure resulting in an intimate apposition of the blastocyst with the uterine luminal epithelium. Expression of EP(3) and FP primarily in the circular muscle of the myometrium on Days 3-5 of pregnancy suggests that the circular muscle, not the longitudinal muscle, is the primary target for PC-mediated uterine contractions required for embryo transport, spacing, and/or accommodation in the uterus. In contrast, expression of EP(3) in a subpopulation of cells in the stromal bed at the mesometrial side, and of EP(4) in the epithelium and stroma on these days, suggests that PGE(2) effects on uterine preparation for implantation (such as epithelial cell differentiation, stromal cell proliferation, uterine edema, luminal closure, and increased localized endometrial vascular permeability at the sites of blastocyst attachment) are mediated by these receptor subtypes. Similar expression patterns of EP(3) and EP(4) in the Day 4 pseudopregnant mouse uterus or in the ovariectomized uterus under combined treatment with estrogen and progesterone suggest that these genes are regulated by ovarian steroids rather than by the embryo during the preimplantation period (Days 1-4). In contrast, the expression of these genes during the postimplantation period (Days 5-8) is associated with the onset of decidualization.zh_CN
dc.language.isoen_USzh_CN
dc.subjectENDOTHELIAL GROWTH-FACTORzh_CN
dc.subjectDECIDUAL CELL REACTIONzh_CN
dc.subjectBLASTOCYST IMPLANTATIONzh_CN
dc.subjectPROSTANOID RECEPTORSzh_CN
dc.subjectEARLY-PREGNANCYzh_CN
dc.subjectMESSENGER-RNASzh_CN
dc.subjectBINDING-SITESzh_CN
dc.subjectRATzh_CN
dc.subjectCLONINGzh_CN
dc.subjectSUBTYPEzh_CN
dc.titlePotential sites of prostaglandin actions in the periimplantation mouse uterus: Differential expression and regulation of prostaglandin receptor geneszh_CN
dc.typeArticlezh_CN


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record