Potential sites of prostaglandin actions in the periimplantation mouse uterus: Differential expression and regulation of prostaglandin receptor genes
Yang, Z. M.
Das, S. K.
Dey, S. K.
- 生命科学－已发表论文 
Prostaglandins (PGs), especially PGE(2) and PGF(2 alpha), are considered important for blastocyst spacing, implantation, and decidualization in the rodent uterus. However, information regarding uterine sites of PG actions in these processes is lacking. PGE(2) or PGF(2 alpha) interacts with specific G protein-coupled membrane receptors. PGE(2) receptors are classified into four subtypes, EP(1), EP(2), EP(3), and EP(4). While EP(1) is coupled to Ca2+ mobilization, activation of EP(2) and EP(4) triggers stimulation of adenylyl cyclase. In contrast, activation of EP(3) inhibits adenylyl cyclase. PGF(2 alpha) receptor (FP) is coupled to stimulation of phospholipase C-inositol trisphosphate (IP3) pathway and Ca2+ mobilization. This investigation demonstrates that PGE(2) and PGF(2 alpha) receptor genes are expressed in a temporal and cell-specific manner in the periimplantation mouse uterus. In the mouse, the attachment reaction occurs in the evening (2200-2300 h) of Day 4 of pregnancy and is preceded by embryo spacing, uterine edema, and luminal closure resulting in an intimate apposition of the blastocyst with the uterine luminal epithelium. Expression of EP(3) and FP primarily in the circular muscle of the myometrium on Days 3-5 of pregnancy suggests that the circular muscle, not the longitudinal muscle, is the primary target for PC-mediated uterine contractions required for embryo transport, spacing, and/or accommodation in the uterus. In contrast, expression of EP(3) in a subpopulation of cells in the stromal bed at the mesometrial side, and of EP(4) in the epithelium and stroma on these days, suggests that PGE(2) effects on uterine preparation for implantation (such as epithelial cell differentiation, stromal cell proliferation, uterine edema, luminal closure, and increased localized endometrial vascular permeability at the sites of blastocyst attachment) are mediated by these receptor subtypes. Similar expression patterns of EP(3) and EP(4) in the Day 4 pseudopregnant mouse uterus or in the ovariectomized uterus under combined treatment with estrogen and progesterone suggest that these genes are regulated by ovarian steroids rather than by the embryo during the preimplantation period (Days 1-4). In contrast, the expression of these genes during the postimplantation period (Days 5-8) is associated with the onset of decidualization.