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dc.contributor.authorMa, Yzh_CN
dc.contributor.authorLin, SQzh_CN
dc.contributor.authorGao, Yzh_CN
dc.contributor.authorLi, Mzh_CN
dc.contributor.authorLuo, WXzh_CN
dc.contributor.authorZhang, Jzh_CN
dc.contributor.authorXia, NSzh_CN
dc.contributor.author张军zh_CN
dc.date.accessioned2013-12-12T02:24:02Z
dc.date.available2013-12-12T02:24:02Z
dc.date.issued2003-10zh_CN
dc.identifier.citationWorld Journal of Gastroenterology, 2003,9(10):2211-2215zh_CN
dc.identifier.issn1007-9327zh_CN
dc.identifier.otherWOS:000186055100018zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65114
dc.description.abstractAIM: To transfer hepatitis E virus (HEV) ORF2 partial gene to tomato plants, to investigate its expression in transformants and the immunoactivity of expression products and to explore the feasibility of developing a new type of plant-derived HEV oral vaccine. METHODS: Plant binary expression vector p1301E2, carrying a fragment of HEV open reading frame-2 (named HEV-E2), was constructed by linking the fragment to a constitutive CaMV35s promoter and nos terminator, then directly introduced into Agrobacterium tumefaciens EHA105. With leaf-disc method, tomato plants medicated by EHA105 were transformed and hygromycin-resistant plantlets were obtained in selective medium containing hygromycin. The presence and integration of foreign DNA in transgenic tomato genome were confirmed by Gus gene expression, PCR amplification and Southern dot blotting. The immunoactivity of recombinant protein extracted from transformed plants was examined by enzyme-linked immunosorbant assay (ELISA) using a monoclonal antibody specifically against HEV. ELISA was also used to estimate the recombinant protein content in leaves and fruits of the transformants. RESULTS: Seven positive lines of HEV-E2-transgenic tomato plants confirmed by PCR and Southern blotting were obtained and the immunoactivity of recombinant protein could be detected in extracts of transformants. The expression levels of recombinant protein were 61.22 ng/g fresh weight in fruits and 6.37-47.9 ng/g fresh weight in leaves of the transformants. CONCLUSION: HEV-E2 gene was correctly expressed in transgenic tomatoes and the recombinant antigen derived from them has normal immunoactivity. Transgenic tomatoes may hold a good promise for producing a new type of low-cost oral vaccine for hepatitis E virus.zh_CN
dc.language.isoen_USzh_CN
dc.subjectB SURFACE-ANTIGENzh_CN
dc.subjectORAL IMMUNIZATIONzh_CN
dc.subjectTRANSGENIC TOBACCOzh_CN
dc.subjectEDIBLE VACCINEzh_CN
dc.subjectLT-Bzh_CN
dc.subjectIMMUNOGENICITYzh_CN
dc.subjectPOTATOzh_CN
dc.subjectGLYCOPROTEINzh_CN
dc.subjectANIMALSzh_CN
dc.subjectPLANTSzh_CN
dc.titleExpression of ORF2 partial gene of hepatitis E virus in tomatoes and immunoactivity of expression productszh_CN
dc.typeArticlezh_CN


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