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dc.contributor.authorWang, Weizh_CN
dc.contributor.authorCheng, Tongzh_CN
dc.contributor.authorMa, Kezh_CN
dc.contributor.authorXia, Dezhenzh_CN
dc.contributor.authorWang, Yongmeizh_CN
dc.contributor.authorLiu, Jianzh_CN
dc.contributor.authorDu, Hailianzh_CN
dc.contributor.authorShih, James Wai Kuozh_CN
dc.contributor.authorZhang, Junzh_CN
dc.contributor.authorZhao, Qinjianzh_CN
dc.contributor.authorXia, Ningshaozh_CN
dc.contributor.author张军zh_CN
dc.date.accessioned2013-12-12T02:24:02Z
dc.date.available2013-12-12T02:24:02Z
dc.date.issued2013-03zh_CN
dc.identifier.citationJournal of virological methods, 2013,188(1-2):114-20zh_CN
dc.identifier.issn1879-0984zh_CN
dc.identifier.otherMEDLINE:23274754zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/65109
dc.description.abstractThe baculovirus expression vector system (BEVS) is one of the most powerful methods for production of recombinant proteins for research or commercial purposes. Titration of viable virus in insect cell culture is often required when BEVS is used for basic research or bioprocessing. An enzyme-linked immunosorbent spot (ELISPOT) assay using monoclonal antibodies against the major capsid protein VP39 of both Autographa californica nuclear polyhedrosis virus (AcMNPV) and Bombyx mori nuclear polyhedrosis virus (BmNPV) was developed for baculovirus quantitation at 48 h post-infection. The titer was determined by visualizing infected insect cells as blue spots and automated spot counting was achieved with ELISPOT hardware and software. Log-scale comparison of the results between the ELISPOT assay and a conventional end point dilution assay using a fluorescent marker showed a good correlation for both AcMNPV (R(=)(2)0.9980,p<0.05) and BmNPV (R-2=0.9834,p<0.05). In conclusion, a novel, rapid and semi-automated procedure for titrating baculovirus was developed based on the specific immunostaining of infected cells followed by automated spot counting. (C) 2012 Elsevier B.V. All rights reserved.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1016/j.jviromet.2012.12.011zh_CN
dc.subjectELISPOTzh_CN
dc.subjectBaculovirus titrationzh_CN
dc.subjectMonoclonal antibodyzh_CN
dc.subjectVP39zh_CN
dc.titleDevelopment of a novel baculovirus titration method using the Enzyme-linked immunosorbent spot (ELISPOT) assayzh_CN
dc.typeArticlezh_CN


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