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dc.contributor.authorJiang, Azh_CN
dc.contributor.authorLi, CYzh_CN
dc.contributor.authorGao, Yzh_CN
dc.contributor.authorZhang, Mzh_CN
dc.contributor.authorHu, Jzh_CN
dc.contributor.authorKuang, WHzh_CN
dc.contributor.authorHao, SCzh_CN
dc.contributor.authorYang, WZzh_CN
dc.contributor.authorXu, CCzh_CN
dc.contributor.authorGao, GQzh_CN
dc.contributor.authorWang, ZCzh_CN
dc.contributor.authorLiu, ZGzh_CN
dc.date.accessioned2013-12-12T02:13:08Z
dc.date.available2013-12-12T02:13:08Z
dc.date.issued2006-12zh_CN
dc.identifier.citationCornea, 2006,25(10):S36-S40zh_CN
dc.identifier.issn0277-3740zh_CN
dc.identifier.otherWOS:000243202600007zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/62758
dc.description.abstractPurpose: To prepare amniotic extraction (AE) and to test its antiangiogenic effect in vivo and in vitro. Methods: AE was prepared and diluted to 50, 100, and 200 mu g/mL concentrations. Alkali burn-induced corneal neovascularization (NV) was produced and topically treated with different concentrations of AE or 0.1% dexamethasone for 7 days. Normal saline was used as a control. Corneal NV was visualized by heart perfusion of Chinese ink and quantified as the percentage of corneal NV area to the whole corneal area. Human umbilical vein endothelial cells (HUVECs) were primarily cultured. The effects of AE on proliferation and tube formation of HUVECs were tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and in vitro angiogenesis assay. Pigment epithelium-derived factor (PEDF) in AE was detected by Western blot. Results: Relative corneal NV area in the control group was 56.6% +/- 9.9%, which was significantly reduced by 50 mu g/mL AE (47.6% +/- 6.9%; P = 0.043) and 200 mu g/mL AE (34.3% +/- 7.8%; P < 0.001) and by 0.1% dexamethasone (21.1% +/- 1.8%; P < 0.001). HUVEC cell proliferation was significantly decreased after treatment with AE at concentrations of 50 and 100 mu g/mL compared with control (P = 0.036 and 0.001, respectively). The tube formation was significantly suppressed by 100 mu g/mL AE (70.03% +/- 4.35%) compared with control (100% +/- 4.84%; P = 0.002). No expression of PEDF was detected in AE. Conclusion: AE inhibits NV induced by alkali burn. This effect may be elicited at least in part through the inhibiting activity of blood vessel endothelial cells and is not associated with AE.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1097/01.ico.0000247211.78391.afzh_CN
dc.subjectCORNEAL NEOVASCULARIZATIONzh_CN
dc.subjectMEMBRANE TRANSPLANTATIONzh_CN
dc.subjectSURFACE RECONSTRUCTIONzh_CN
dc.subjectRISK-FACTORSzh_CN
dc.subjectSUPPRESSIONzh_CN
dc.subjectIDENTIFICATIONzh_CN
dc.subjectCULTUREzh_CN
dc.subjectFAILUREzh_CN
dc.subjectCELLSzh_CN
dc.titleIn vivo and in vitro inhibitory effect of amniotic extraction on neovascularizationzh_CN
dc.typeArticlezh_CN


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