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dc.contributor.authorZheng, Jiangzh_CN
dc.contributor.authorHao, Jumingzh_CN
dc.contributor.authorZi, Huaishuzh_CN
dc.contributor.authorLi, Yubaozh_CN
dc.contributor.authorYan, Qingpizh_CN
dc.contributor.authorWang, Junzh_CN
dc.contributor.authorSu, Yongquanzh_CN
dc.contributor.author苏永全zh_CN
dc.date.accessioned2013-12-12T02:08:39Z
dc.date.available2013-12-12T02:08:39Z
dc.date.issued2011-10zh_CN
dc.identifier.citationSensor Letters, 2011,9(5):2017-2020zh_CN
dc.identifier.issn1546-198Xzh_CN
dc.identifier.otherWOS:000301155100073zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/60669
dc.description.abstractSystematic evolution of ligands by exponential enrichment (SELEX) is a new technique that can screen high affinity oligonucleotide probe, called aptamer which have been widely used in the detections of mciroorganism, proteins, tumor cells and so on. However, as a key step in SELEX process, the affinity determination method is variable for the different targets. In the present paper, an aptamer against Vibrio alginolyticus was used to study and optimize the affinity determination method for the vibrio detection. Based on biotin-avidin system marked by enzyme, the binding temperature, time and binding ratio (vibro/aptamer) were studied and optimized through orthogonal experiment. The results showed that the ideal affinity was obtained after the aptamer bound with V.alginolyticus for 70 minutes at the temperature 32 degrees C by a mole ratio 1:100 (vibrio:aptamer). By this optimal affinity detemination method, the aptamer was used to test the affinity with four kinds of microorganisms and the result showed that the aptamer had the highest affinity with V.alginolyticus, which suggesting its specificity of the aptamer against V.alginolyticus.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1166/sl.2011.1527zh_CN
dc.subjectIN-VITRO SELECTIONzh_CN
dc.subjectACID APTAMERSzh_CN
dc.subjectSELEXzh_CN
dc.titleAffinity Determination Method in Vibrio Detection Based on Aptamer-An Oligonucleotide Probe Screened by Systematic Evolution of Ligands by Exponential Enrichmentzh_CN
dc.typeArticlezh_CN


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