Study on Vibrio Disease of Pseudosciaena crocea and Recognition of the Disease Resistant Group Based on Automatic Detection System for Hemogram

Abstract

Chemical medicines and antibiotics can kill pathogenic microorganisms in aquiculture animals, but they will lead to environmental pollution and serious food safety problems. Therefore, study on the selection for the disease resistant group of animals is necessary to solve the problem and promote the development of aquaculture. From the view of the relationship between fishes and pathogenic microorganisms, the present paper studies hemogram changes of Pseudosciaena crocea with different disease resistant abilities after artificial infected with the Vibrio harveyi. The results showed that the changes of hemograms of Pseudosciaena crocea after infected with Vibrio harveyi had close relationship with its disease resistant abilities. The hemogram indicators of the high disease resistant group (HDRG) did not change remarkably after infection compared with the control (P >0.05). But in the low disease resistant group (LDRG) or sensitive group, the concentrations of the red blood cells, white blood cells, lymphocytes, monocytes and neutrophil cells, as well as hematocrit and hemoglobin content per litre blood, decreased remarkably compared with the control (p < 0.05). And the white blood cells, monocytes and neutrophil cells of the LDRG also decreased remarkably compared with that of the HDRG (P < 0.05). These indicators of hemogram provide better referrences for the study on the anti-disease ability of Pseudosciaena crocea, and also provide selection basis for the group with high disease resistant abilities. Application of automatic detection system greatly improved the analytical efficiency and would be a better choice for a large number of samples.