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dc.contributor.authorXu, Y.zh_CN
dc.contributor.authorYe, H. H.zh_CN
dc.contributor.authorMa, J.zh_CN
dc.contributor.authorHuang, H. Y.zh_CN
dc.contributor.authorWang, G. Z.zh_CN
dc.contributor.author王桂忠zh_CN
dc.date.accessioned2013-12-12T02:08:36Z
dc.date.available2013-12-12T02:08:36Z
dc.date.issued2010-09zh_CN
dc.identifier.citationIn Vitro Cellular & Developmental Biology-Animal, 2010,46(8):708-717zh_CN
dc.identifier.issn1071-2690zh_CN
dc.identifier.otherISI:000281396600008zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/60628
dc.descriptionNational Natural Science Foundation of China [30300269, 40776084]; Fujian Province Universityzh_CN
dc.description.abstractCrustacean neurons, obtained from the cerebral ganglion of the mud crab Scylla paramamosain, were successfully cultured in vitro. They maintained typical morphological characteristics and showed better outgrowth in modified Medium 199 (M199) medium than that in Liebowitz's L-15 medium. Fetal bovine serum (FBS), muscle extracts, and hemolymph of the mud crab S. paramamosain were added as supplements. Only 20% FBS could promote neuron outgrowth, while muscle extracts and hemolymph of S. paramamosain did not improve neuron outgrowth. For cell dissociation, both collagenase type I and trypsin worked well as determined by initial cell viability and following cell outgrowth potential. More than six kinds of cells with different morphological characteristics were identified in the neuron outgrowth. They were "small cells", "veilers", "branchers", "multipolar cells", "super-large cell", and "bipolar cells". Among all of the cells, bipolar cells were identified for the first time in crustacean neurons culture and they could live longer than other cells. The neurons could grow for more than a week before retraction and eventual degradation.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1007/s11626-010-9327-xzh_CN
dc.subjectPRIMARY-CELL CULTUREzh_CN
dc.subjectUCA-PUGILATORzh_CN
dc.subjectFIDDLER-CRABzh_CN
dc.subjectPEPTIDERGIC NEURONSzh_CN
dc.subjectINVITROzh_CN
dc.subjectBRAINzh_CN
dc.subjectCHINENSISzh_CN
dc.subjectBALANCEzh_CN
dc.subjectTISSUESzh_CN
dc.subjectINVIVOzh_CN
dc.titlePrimary culture and characteristic morphologies of neurons from the cerebral ganglion of the mud crab, Scylla paramamosainzh_CN
dc.typeArticlezh_CN


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