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dc.contributor.authorLiu, J. W.zh_CN
dc.contributor.authorJiao, N. Z.zh_CN
dc.contributor.authorCai, H. N.zh_CN
dc.contributor.authorHong, H. S.zh_CN
dc.contributor.author焦念志zh_CN
dc.date.accessioned2013-12-12T02:08:27Z
dc.date.available2013-12-12T02:08:27Z
dc.date.issued2007zh_CN
dc.identifier.citationActa Oceanologica Sinica,26(4):123-131zh_CN
dc.identifier.issn0253-505Xzh_CN
dc.identifier.otherISI:000249470200013zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/60503
dc.description.abstractPCNA has been considered as a useful marker for the estimation of growth rates of marine phytoplankton at the species level. Since dinoflagellates are noted for having many prokaryotic features in that they are the only eukaryotes to have permanently condensed chromosomes as well as lacking histones and a nucleosome, the sensitivity to UVB radiation and the validity of PCNA as a maker of growth rate in dinoflagellate need to be evaluated. Prorocentrum donghaiense Lu was investigated to valuate the UVB sensitivity in relation to cellular and molecular aspects of PCNA as a growth indicator. The effects of UVB radiation on PCNA were studied using the methods of western blots technology and whole-cell immunoflurescence with one mono-antibody. UVB changed the cell morphology, halted the growth and increased the cell size, even caused cell death to a certain extent after treatment with UVB radiation in P. donghaiense. Compared with the control, treating the algal cultures in exponential phases with UVB radiation for 24 h caused chromatin release and increases in protein levels of PCNA.zh_CN
dc.language.isoen_USzh_CN
dc.subjectULTRAVIOLET-B RADIATIONzh_CN
dc.subjectMARINE-PHYTOPLANKTONzh_CN
dc.subjectOZONE DEPLETIONzh_CN
dc.subjectANTARCTIC PHYTOPLANKTONzh_CN
dc.subjectDNA-DAMAGEzh_CN
dc.subjectBACILLARIOPHYCEAEzh_CN
dc.subjectPHOTOSYNTHESISzh_CN
dc.subjectPROTEINSzh_CN
dc.subjectGENEzh_CN
dc.titleEffects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Luzh_CN
dc.typeArticlezh_CN


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