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dc.contributor.author孙莉萍zh_CN
dc.contributor.authorSun Li-Pingzh_CN
dc.contributor.author张建锋zh_CN
dc.contributor.authorZhang jian-Fengzh_CN
dc.contributor.author李辉zh_CN
dc.contributor.authorLi Huizh_CN
dc.contributor.author王秀燕zh_CN
dc.contributor.authorWang Xiu-Yanzh_CN
dc.contributor.author张召武zh_CN
dc.contributor.authorZhang Zhao-Wuzh_CN
dc.contributor.author王霜zh_CN
dc.contributor.authorWang Shangzh_CN
dc.contributor.author张其清zh_CN
dc.contributor.authorZhang Qi-Qingzh_CN
dc.date.accessioned2013-12-12T02:03:20Z
dc.date.available2013-12-12T02:03:20Z
dc.date.issued2009-01-10zh_CN
dc.identifier.citation高等学校化学学报, 2009,30(1):95-99zh_CN
dc.identifier.issn0251-0790zh_CN
dc.identifier.otherISI:000262921100022zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/59261
dc.description.abstract【中文摘要】研究了金纳米粒子与单链DNA在不有PH值时的相互作用以及金纳米粒子与不同碱基序列单链DNA的相互作用。结果表明,在PH为12.6的强碱性条件下,单链DNA能使金纳米粒子稳定分散在溶液中;在PH为1.4的强酸性条件下,单链DNA能保护金纳米粒子不发生融合,而只发生团聚,且团聚现象具有可逆性。不同寡核苷酸对金纳米粒子的亲和力按poly dA>poly dC>poly dT的顺序依次减弱。单链DNA对纳米金的保护作用强度与单链DNA的长度成正比。【Abstract】The interaction between unmodified gold nanoparticles and single-stranded DNA (ssDNA) was studied at different pH value. And the sequence-dependent stability of ssDNA-GNPs complex was investigated. GNPs precipitated from red colloid solution in the form of aggregation after alkalization with NaOH or dissolve after acidification with HCL Both processes were irreversible. If the same particles were incubated with ssDNA, ssDNA-GNPs complex were stable against NaOH-induced aggregation at pH = 12.6, and aggregated at pH = 1.4, while redispersed at pH = 12.6. It was concluded that unmodified GNPs could be coated by unmodified ssDNA, which protected GNPs dispersing in solution at pH = 12.6, and prevented GNPs front dissolving at pH = 1.4, as measured by TEM and UV-Vis absorption spectrum. The bindig affinities of oligonucleotides to GNPs were different in the order of poly dA > poly dC > poly dT. Moreover, longer ssDNA had stronger protective effect to gold nanoparticles. HCl-induced GNPs aggregation could be an effective method to identify the diversity of deoxyribonucleotides in ssDNA sequences.zh_CN
dc.language.isoen_USzh_CN
dc.subject金纳米粒子zh_CN
dc.subjectGold nanoparticlezh_CN
dc.subject单链DNAzh_CN
dc.subjectSingle-stranded DNAzh_CN
dc.subject相互作用zh_CN
dc.subjectInteractionzh_CN
dc.subjectssDNAzh_CN
dc.title金纳米粒子与单链DNA的相互作用zh_CN
dc.title.alternativeInteraction Between Gold Nanoparticles and ssDNAzh_CN
dc.typeArticlezh_CN


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