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dc.contributor.authorYe, Shefangzh_CN
dc.contributor.authorWang, Yiwangzh_CN
dc.contributor.authorJiao, Feizh_CN
dc.contributor.authorZhang, Honggangzh_CN
dc.contributor.authorLin, Cuilinzh_CN
dc.contributor.authorWu, Yihuizh_CN
dc.contributor.authorZhang, Qiqingzh_CN
dc.contributor.author叶社房zh_CN
dc.date.accessioned2013-12-12T02:03:18Z
dc.date.available2013-12-12T02:03:18Z
dc.date.issued2011-05zh_CN
dc.identifier.citationJournal of Nanoscience and Nanotechnology, 2011,11(5):3773-3781zh_CN
dc.identifier.issn1533-4880zh_CN
dc.identifier.otherWOS:000290692400006zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/59233
dc.descriptionNatural Science Foundation of China [30901175]; Science and Technology Innovation Project of Fujian Province for Young Scientific Researchers, China [2008F3097]zh_CN
dc.description.abstractRecent studies suggest reactive oxygen species (ROS) induced in mammalian cells exposed to multi-walled carbon nanotubes (MWCNTs) could mediate the cytotoxicity. This study was conducted to determine the mechanisms responsible for MWCNTs-induced ROS production in human primary macrophages. Our results showed that superoxide levels were significantly increased in a time-dependent manner in blood monocyte-derived macrophages treated with 100 mu g/ml MWCNTs for 12 h. Concomitantly, MWCNTs induced membrane translocation of the NADPH oxidase subunits p47(phox) and p67(phox), a signature event for NADPH oxidase activation. Pre-incubation with apocynin, a selective inhibitor of NADPH oxidase, prevented both membrane translocation of p47(phox) and superoxide production. Treatment with MWCNTs also resulted in an increased cytotoxicity in human primary macrophages that was significantly attenuated by both apocynin and antioxidants. These findings demonstrate that MWCNTs activate NADPH oxidase in human macrophages, which may contribute to ROS generation in MWCNTs treated-macrophages.zh_CN
dc.language.isoen_USzh_CN
dc.source.urihttp://dx.doi.org/10.1166/jnn.2011.3862zh_CN
dc.subjectC57BL/6 MICEzh_CN
dc.subjectIN-VITROzh_CN
dc.subjectKAPPA-Bzh_CN
dc.subjectCELLSzh_CN
dc.subjectEXPOSUREzh_CN
dc.subjectLUNGzh_CN
dc.subjectDNAzh_CN
dc.subjectNANOPARTICLESzh_CN
dc.subjectACCUMULATIONzh_CN
dc.subjectMECHANISMzh_CN
dc.titleThe Role of NADPH Oxidase in Multi-Walled Carbon Nanotubes-Induced Oxidative Stress and Cytotoxicity in Human Macrophageszh_CN
dc.typeArticlezh_CN


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