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  • 2007年第13卷
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血红蛋白在月桂酸修饰玻碳电极的电催化行为
Voltammetric Behavior and Electrocatalytic Properties of Hemoglobin at Glassy Carbon Electrode Modified with Lauric Acid

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Date
2007-05-28
Author
苗新蕊
张旭红
谢英
MIAO Xin-rui
ZHANG Xu-hong
XIE Ying
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  • 2007年第13卷 [85]
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Abstract
研究血红蛋白在月桂酸修饰电极上的电化学行为,在0.02mol.L-1KH2PO4-Na2HPO4(pH=7)的缓冲液中,+0.6~-0.7V(vs.Ag/AgCl)电位范围内,Hb于该修饰电极产生不可逆还原电流峰.还原峰电流ip与v1/2呈线性关系,ip随溶液pH值和血红蛋白浓度的增加而增大,其浓度在1.00×10-8~5.00×10-9mol.L-1和1.92×10-6~2.06×10-7mol.L-1范围内分段呈线性变化关系.实验数据经进一步分析拟合,得到更精确的信息.该电极可作为检测血红蛋白的新型电化学生物传感器.
 
The electrochemical behavior of hemoglobin at glassy carbon(GC)electrode modified with Lauric Acid was studied.In the potential of +0.6~-0.7 V(vs.Ag/AgCl),an irreversible cathodic peak was observed at-0.3 V at the scan rate of 20 mV·s-1.In the medium of 0.02 mol·L-1 KH2PO4-Na2HPO4(pH 7.0),ip increased with the increasing concentration of Hb and the value of pH.The peak currents were proportional to the concentrations of hemoglobin in the range of 1.00×10-8~5.00×10-9 mol·L-1 and 1.92×10-6~2.06×10-7 mol·L-1.The data was analyzed in details by simulating in order to obtain more information and reaction mechanism of Hb on modified electrode.The electrode can be used as an approach for determining hemoglobin with high sensitivity.
 
Citation
电化学,2007,13(02):203-206.
URI
https://dspace.xmu.edu.cn/handle/2288/58095

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