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dc.contributor.author许娟zh_CN
dc.contributor.author黄桂萍zh_CN
dc.contributor.author李红zh_CN
dc.contributor.author朱伟zh_CN
dc.contributor.authorXU Juanzh_CN
dc.contributor.authorHUANG Gui-pingzh_CN
dc.contributor.authorLI Hongzh_CN
dc.contributor.authorZHU Weizh_CN
dc.date.accessioned2013-11-18T07:46:12Z
dc.date.available2013-11-18T07:46:12Z
dc.date.issued2007-11-28zh_CN
dc.identifier.citation电化学,2007,13(04):372-376.zh_CN
dc.identifier.issn1006-3471zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/58035
dc.description.abstract应用循环伏安法、微分脉冲伏安法和荧光光谱法研究了鲱鱼精DNA的电化学氧化及其与组蛋白的相互作用.结果发现,在0.20~1.25 V电位区间内,DNA在酸性溶液中呈现一个明显的不可逆氧化峰,在中性及碱性溶液中呈现两个不可逆氧化峰.氧化峰电位随溶液pH值增大而负移,变化幅度为-57 mV.pH-1.氧化峰电流与DNA浓度(0.45~8.20 mmol.L-1)成线性关系.DNA能与组蛋白结合,导致氧化电位正移,氧化电流减小,并减弱钌配合物指示剂和DNA相互作用的荧光强度以及减少DNA的氧化损伤.zh_CN
dc.description.abstractThe voltammetric behavior of herring sperm DNA at glassy carbon electrode and its interaction with histone have been investigated by using cyclic voltammetry,differential pulse voltammetry,and fluorescence measurement.In the potential range of 0.20 V to 1.25 V,it is found that an irreversible oxidative peak is exhibited in acid solution,but in the neutral or alkaline solution two oxidative peaks are observed.Oxidative peak potential values show a negative shift with increasing pH via a slope of-57 mV·pH-1 and a linear relation with DNA concentration in the range of 0.45 to 8.20 mmol·L-1.In the presence of histone,the oxidative peak current of DNA and the fluorescence intensity of interactive product between a ruthenium complex and DNA are found to have an obvious decrease and the oxidative potentials show a positive shift.Moreover,the experimental results suggest that the DNA damage is weakened based on its binding interaction with histone.zh_CN
dc.language.isozhzh_CN
dc.publisher厦门大学《电化学》编辑部zh_CN
dc.relation.ispartofseries研究论文zh_CN
dc.relation.ispartofseriesArticleszh_CN
dc.source.urihttp://electrochem.xmu.edu.cn/CN/abstract/abstract8670.shtmlzh_CN
dc.subject鲱鱼精DNAzh_CN
dc.subject电化学氧化zh_CN
dc.subject组蛋白zh_CN
dc.subject相互作用zh_CN
dc.subjectherring sperm DNAzh_CN
dc.subjectelectrochemical oxidationzh_CN
dc.subjecthistonezh_CN
dc.subjectinteractionzh_CN
dc.title鲱鱼精DNA的电化学氧化及与组蛋白的相互作用zh_CN
dc.title.alternativeElectrochemical Oxidization of Herring Sperm DNA and its Interaction with Histonezh_CN
dc.typeArticlezh_CN
dc.description.note作者联系地址:华南师范大学化学与环境学院,华南师范大学化学与环境学院,华南师范大学化学与环境学院,广州市疾病预防控制中心毒理科 广东广州510006,广东广州510006,广东广州510006,广东广州510080zh_CN
dc.description.noteAuthor's Address: 1.School of Chemistry and Environment,South China Normal University,Guangzhou 510006,China2Unit of Molecular Toxicology,Center for Disease Control and Prevention,Guangzhou 510080,Chinazh_CN


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