Show simple item record

dc.contributor.author曹晓卫zh_CN
dc.contributor.author杨海峰zh_CN
dc.contributor.author王浙苏zh_CN
dc.contributor.author孟晓云zh_CN
dc.contributor.author吴霞琴zh_CN
dc.contributor.author章宗穰zh_CN
dc.contributor.authorCAO Xiao-Weizh_CN
dc.contributor.authorYANG Hai-Fengzh_CN
dc.contributor.authorWANG Zhe-Suzh_CN
dc.contributor.authorMENG Xiao-Yunzh_CN
dc.contributor.authorWU Xia-Qinzh_CN
dc.contributor.authorZHANG Zong-Rang
dc.date.accessioned2013-11-18T07:44:55Z
dc.date.available2013-11-18T07:44:55Z
dc.date.issued2001-02-28zh_CN
dc.identifier.citation电化学,2001,7(01):52-54.zh_CN
dc.identifier.issn1006-3471zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/57627
dc.description.abstract本文概述了采用电化学现场拉曼光谱技术研究氧化物歧化酶在L 半胱氨酸修饰金电极表面的电子迁移反应以及腺嘌呤共存条件下超氧化物歧化酶在金电极表面的电子迁移反应和不同电位下银电极表面烟酰胺腺嘌呤二核苷酸的吸附等体系的反应吸附特性 .所得结果对于分析和研究生物活性分子电化学过程机理具有重要意义 .zh_CN
dc.description.abstractElectron?transfer reaction is known to be one of the key reactions for generating biological functions. Mechanism revelation at a molecular level of such kind reactions is to be very helpful for us to understand life essence. In fact, surface enhanced Raman scattering (SERS) is one of the most powerful tools for the study on metal?electrolyte and metal?vacuum interfaces since 1970's. Moreover, Raman spectroscopic study in enzymology has provided attractive results during last twenty?five years. For the study of electron?transfer reaction mechanism of some oxidoreductases and SERS of some other biological macromolecules, an electrochemical in situ Raman spectroscopic technique was established in author's lab and some research works have been done on it in the past two years. A brief review of these works is given in this paper. The electrochemical in situ Raman spectroscopic measurements were carried out using a Super LABRAM Raman spectrometer (Dilor, France) coupled with a CHI604A Electrochemical Analyzer (CH Instr., USA). A Teflon spectroelectrochemical cell with a quartz plate window was designed for the in situ measurements. The working electrode was pretreated with oxidation?reduction cycles for each measurement. The electrolyte solutions were purged with nitrogen prior to all measurements, and all the measurements were carried out under the nitrogen atmosphere. Copper, zinc superoxide dismutase (SOD) is an important oxidoreductase for organism metabolism. The established spectroelectrochemical technique was first used to characterize the cyclic voltammetric process of SOD at L?cysteine modified gold electrode as well as the process of electrochemical modification of L?cysteine molecules on a gold electrode. The obtained Raman spectra reveal that the L?cysteine modified gold electrode improves effectively the reversibility of electron?transfer reactions of SOD. Besides L?cysteine molecules, it was interesting that adenine was also an effective electron?transfer promoter for SOD at gold electrode. A strong peak at 355 cm -1 can be observed in the Raman spectrum of adenine molecules adsorbed on gold electrode. It was inferred that the peak maybe related to the chemical interaction between adenine molecules adsorbed and gold electrode surface. As shown in Fig.1, for the mixture of SOD and adenine at gold electrode under a polarization potential 55 mV (vs. SCE), both the characteristic Raman lines of SOD and adenine molecules appeared. Therefore it was reasonable to conclude that SOD and adenine molecules should be co?adsorbed on gold electrode surface under such a potential, which is slightly lower than the reduction peak potential of SOD on adenine?modified gold electrode. Moreover, two new peaks appeared remarkably at 445 cm -1 and 610 cm -1 are likely to be related to the active site of SOD. It suggests that the co?adsorption mechanism of SOD and adenine molecules on the gold electrode surface results in effective approaching of the active site of SOD to the electrode surface.zh_CN
dc.language.isozhzh_CN
dc.publisher厦门大学《电化学》编辑部zh_CN
dc.relation.ispartofseries研究论文zh_CN
dc.relation.ispartofseriesArticleszh_CN
dc.source.urihttp://electrochem.xmu.edu.cn/CN/abstract/abstract9284.shtmlzh_CN
dc.subject生物分子zh_CN
dc.subject电化学现场zh_CN
dc.subject拉曼光谱学zh_CN
dc.subjectBioactive moleculeszh_CN
dc.subjectElectrochemical in situzh_CN
dc.subjectRaman spectroscopyzh_CN
dc.title生物活性分子的拉曼光谱电化学研究(英文)zh_CN
dc.title.alternativeRaman Spectroelectrochemical Study on Bioactive Moleculeszh_CN
dc.typeArticlezh_CN
dc.description.note作者联系地址:上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234,上海师范大学化学系!上海200234zh_CN
dc.description.noteAuthor's Address: Dept. of Chem., Shanghai Teachers Univ., Shanghai 200234,Chinazh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record