Influence of o-Phenylenediamine on the Catalysis at HRP Enzyme Electrode in l,4-Dioxane
- 1995年第1卷 
邻苯二胺对１，４－二氧六环介质中ＨＲＰ酶电极催化性能的影响陈红，吴辉煌（厦门大学固体表面物理化学国家重点实验室，厦门大学化学系，厦门３６１００５）１实验部分辣根过氧化物酶（ＨＲＰ，Ｅ．Ｃ．１．１１．１．７，２５０ｕ／ｍｇ，Ｒ．Ｚ．～３．０）及牛血清蛋...Horseradish peroxidase(HRP)was immobilized by crosslinkage on the glassycarbon electrode to prepare the enzyme electrede ,where the reduction of H_2O_2 was studied in l,4-dioxane media. It was shown that HRP electrode can catalyze the reduction of H_2O_2 without anymedintor,however, as an activator,o-phenylenediamine can promote the bio-catalysis. In thepresence of the activator,the parallel reactions occurred on the enzyme electrode. The workingcondition of the HRP electrede was investigated as the sensors for H_2O_2 and o-phenylenediamine,alsothe influence of water content in organic phase was discussed on the performance of the enzymeelectrode in organic phase.