乙酰辅酶A羧化酶α-CT亚基accA基因表达载体的构建及在杜氏藻中超量表达的研究
Construction a Recombined Vector that Expressed the accA Gene of α-CT Subunit of Acetyl-CoA Carboxylase and its Over-expression in Dunaliella bardawil
Date
2009-12-21Collections
- 生命科学－学位论文 [2445]
Abstract
生物质能源的开发利用是缓解我国能源和环境压力，建立可持续发展能源系统的有效措施。在众多生物中，藻类具有生物量大、生长周期短、易培养以及脂类含量高等特点，是制备生物质能源的良好材料。本实验室选择杜氏藻(Dunaliellabardawil)作为研究对象，对杜氏藻进行分子生物学改造，将外源编码脂肪合成关键酶——乙酰辅酶A羧化酶(ACCase)的α-CT亚基accA基因整合到杜氏藻染色体上，并让目的蛋白大量表达，以期提高杜氏藻该酶合成脂类的能力，从而获得脂类含量高并且能够稳定遗传的杜氏藻株。 本实验克隆了大肠杆菌ACCase的accA亚基，并成功构建原核表达载体pGEX-6P-1-accA，将载体... It is effective to relieve the pressure from conventional energy shortage and environment and establish the sustainable energy system by exploring and using biomass. The exploitation and utilization of biomass energy are effective stratagems, which not only relieve the pressure from conventional energy shortage and environment, but also establish the sustainable energy system. Among numerous organ...