• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Multiplex immunodetection of tumor markers with a suspension array built upon core-shell structured functional fluorescence-encoded microspheres

Thumbnail
Full Text
Multiplex immunodetection of tumor markers with a suspension array built upon core-shell structured functional fluorescence-encoded microspheres.htm (692bytes)
Date
2010-03
Author
Long, Yao
Zhang, Zhiling
Yan, Xiaomei
颜晓梅
Xing, Jinchun ( First Hosp Xiamen)
Zhang, Kaiyan ( First Hosp Xiamen)
Huang, Jingxiong ( First Hosp Xiamen)
Zheng, Jiaxin ( First Hosp Xiamen)
Li, Wei ( First Hosp Xiamen)
Collections
  • 化学化工-已发表论文 [14469]
Show full item record
Abstract
A new suspension array built upon laboratory-prepared functional fluorescence-encoded polystyrene beads (FFPBs) was developed for multiplex immunodetection of tumor markers. The FFPBs were synthesized by copolymerizing rhodamine 6G (R6G) and carboxyl function groups on the surface of the seed beads forming a core-shell structure. The fabrication process was facile and the encoding fluorescence intensity of the beads can be precisely controlled by adjusting the quantity of R6G. In present work, we demonstrated that the quantity variation of impregnated R6G had negligible effect on the coupling efficiency of biomolecules onto the surface of the FFPBs. The R6G encoding fluorescence remained good monodispersity upon capture probe coupling and immunocomplex formation. No fluorescence resonance energy transfer was observed between the R6G doped in the bead shell and fluorophore used for antibody labeling. Under the optimal conditions, the proposed suspension array allowed simultaneous detection of alpha-fetoprotein, carcinoembryonic antigen, and prostate specific antigen in the ranges of 0.07-500 ng mL(-1), 1-2000 ng mL(-1), and 0.5-500 ng mL(-1), respectively, with detection limits of 0.0626 ng mL(-1), 0.554 ng mL(-1), and 0.250 ng mL(-1). Test on clinical serum samples demonstrated that the results obtained with suspension array were in good agreement with those of the reference electrochemiluminescence immunoassay method. We conclude that the laboratory-made FFPBs are sufficient as the microcarrier for the construction of suspension array in clinical diagnosis. (C) 2010 Elsevier B.V. All rights reserved.
Citation
Analytica Chimica Acta,2010,665(1):63-68
URI
http://dx.doi.org/doi:10.1016/j.aca.2010.03.009
https://dspace.xmu.edu.cn/handle/2288/3731

Related items

Showing items related by title, author, creator and subject.

  • ECFA下闽台经贸关系发展研究 

    邢承 (2011-06-24)
    随着世界经济全球化和区域经济一体化进程的不断加快,许多国家和地区对于建立自由贸易区、实现双边或多边贸易自由化的呼声越来越高。自由贸易区有利于消除贸易障碍、减免关税、降低进出口成本、加速区域经济融合、增强区域互补性等。继“东盟10+1”合作协议签署后,中国大陆与台湾于2010年6月29日正式签署了ECFA(即《海峡两岸经济合作框架协议》,下同)。ECFA是两岸间类似于自由贸易协定的经济合作协议,它的签署标志着两岸的经济合作进入了正常化、制度化的阶段。 ...
  • 一流本科教育的特征与实践走势 

    别敦荣 (2017)
    “双一流”建设是我国高等教育发展的最新国策,是缩小我国与先进国家在高等教育方面差距的重大举措。毫无疑问,我国与先进国家之间的最大差距在于大学的人才培养,发展一流的本科教育是推进高等教育现代化、实现建设高等教育强国发展目标的根本要求。在当前的“双一流”建设中,很多大学主要考虑的还是学科建设和科学研究,而较少重视本科教育,这是短视的。没有一流的本科教育,“双一流”建设将根基不稳。探讨国内外一流本科教育的特征和实践动向,有助于加强“双一流”建设。
  • T型石墨烯及其衍生物的结构与电子特性 

    刘慧英; 张秀钦; 方艺梅; 朱梓忠 (2017-08-20)
    采用基于密度泛函理论的第一原理方法研究了T型石墨烯及其衍生物-n(n=1-5)的结构稳定性和电子结构性质.T型石墨烯是一种拥有四角形环的二维碳材; 料同素异构体,通过改变连接四角形环的碳链上的碳原子个数n,可以得到一系列的sp-sp~2杂化结构,称其为T型石墨烯衍生物-n.计算结果表明:这些; 材料的结构稳定性、化学键类型和电子结构性质都依存于n的奇偶性.其中T型石墨烯(n=0)的结构最稳定,并形成一个由8个碳原子构成的大环.声子谱计算; ...

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies