Quantum Dot-Based Immunochromatographic Fluorescent Biosensor for Biomonitoring Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos
Du, Dan（Pacific NW Natl Lab, Richland）
Wang, Jun（Pacific NW Natl Lab, Richland）
Smith, Jordan N.（Pacific NW Natl Lab, Richland）
Timchalk, Charles（Pacific NW Natl Lab, Richland）
Lin, Yuehe（Pacific NW Natl Lab, Richland）
- 化学化工－已发表论文 
A novel and portable fluorescent sensor that integrates an immunochromatographic test strip assay (ITSA) with a quantum dot (QD) label and a test strip reader was described in this study for simple, rapid, and sensitive biomonitoring of an organophosphorus pesticide metabolite. The principle of this sensor is based on a competitive immunoreaction that was performed on an immunochromatographic test strip, where analytes compete with competitors (QD-conjugated analogs) to bind to antibodies on a test zone. Captured QDs serve as signal vehicles for fluorescent readout. In this work, 3,5,6-trichloropyridinol (TCP) is used as a model analyte to demonstrate the performance of the immunosensor. QD-TCP conjugates were synthesized and characterized with X-ray photoelectron spectroscopy (XPS) and fluorescence spectroscopy. Some parameters (e.g., the amount of QD-modified TCP and immunoreaction time) that govern sensitivity and reproducibility of ITSA were optimized. Under optimal conditions, the sensor has a wide dynamic range and is capable of detecting a minimum 1.0 ng/mL TCP standard analyte in 15 min. The sensor has been successfully applied for detection of TCP spiked in rat plasma with average recovery of 102.0%. Results demonstrate that this sensor provides a rapid, clinically accurate, and quantitative tool for TCP detection and shows great promise for in-field and point-of-care (POC) quantitative testing and screening for metabolite biomarkers, e.g., TCP, for humans exposed to pesticides.
CitationAnal. Chem., 2010, 82 (12)：5125–5133
Showing items related by title, author, creator and subject.
The influence of nanoflagellates on the spatial variety of picoplankton and the carbon flow of the microbial food web in the oligotrophic subtropical pelagic continental shelf ecosystem Chiang, Kuo-Ping; Tsai, An-Yi; Tsai, Pei-Jung; Gong, Gwo-Ching; Huang, Bang-Qin; Tsai, Sheng-Fang; 黄邦钦 (PERGAMON-ELSEVIER SCIENCE LTD, 2014-06-01)To investigate the mechanism of the spatial dynamics of picoplankton community (bacteria and Synechococcus spp.) and to estimate the carbon flux of the microbial food web in the oligotrophic Taiwan Warm Current Water of ...
李文元 (2018-04-10)研究发现在1989年前后北太平洋发生了一次气候突变，而这次气候突变与北太平洋涡旋振荡(NPGO)增强有关。本文利用卫星观测资料和大气海洋再分析资料，对比不同时期北太平洋大气海洋各物理要素的主要变化特征，分析1989年气候突变的特征及NPGO在其中所起的作用，并探讨其与热带太平洋及黑潮延伸体(KE)海气耦合的关系。 结果显示在1989-2004年，东北太平洋SSHAEOF第一和第二模态发生了明显的反转，第一模态变为NPGO模态，该时间段东 ...
Seasonal and spatial patterns of picophytoplankton growth, grazing and distribution in the East China Sea Guo, C.; Liu, H.; Zheng, L.; Song, S.; Chen, B.; Huang, B.; 黄邦钦 (COPERNICUS GESELLSCHAFT MBH, 2014)Dynamics of picophytoplankton population distribution in the East China Sea (ECS), a marginal sea in the western North Pacific Ocean, were studied during two cruises in August 2009 (summer) and January 2010 (winter). ...