Comparison Analysis of Proteome of Both Cerebral and Optic Ganalions in Sepia esculenta
- 化学化工－已发表论文 
Sepia esculenta vests in a cephalopoda animal in mollusca. The differentitation of neural system show not only relative higher level in Cephalopoda animal, but also is easily to separate for studying In the neural sciences. Here, proteomes of both cerebral and optic ganglions in Sepia esculenta were effectively separated by a improved approach of 2D-PAGE. In addition, we used both peptide mass finger printing (PMF) and database search to identify protein spots in part in 2D-PAGE gel for establishing maps of molecular anatomic of cerebral and optic ganglions primarily in Sepia esculenta. We used a software of Melanie 4 Trial to analyze those ganglions, indicating approximately 682 and 594 protein spots in cerebral and optic ganglions, respectively, which the spot numbers in front more than that in later. Moreover, the same proteins such as mitochondrial malate dehydrogenase precursor, pre-MDH, SNAP-type proteins can be found by both ganglions. In addition, these spots such as elongation factor G, tubulin, and actin shows high match rate, but most protein identified in the gel are hypothetic and structural proteins.