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dc.contributor.author魏玲zh_CN
dc.contributor.author杨黄浩zh_CN
dc.contributor.author陈小兰zh_CN
dc.contributor.author曲会英zh_CN
dc.contributor.author许金钩zh_CN
dc.date.accessioned2011-04-26T08:04:33Z
dc.date.available2011-04-26T08:04:33Z
dc.date.issued2003-01zh_CN
dc.identifier.citation高等学校化学学报,2003(1):43-45zh_CN
dc.identifier.issn0251-0790zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/2926
dc.description.abstractA novel fluorimetric method has been developed for the rapid determination of ncleic acids, based on that the catalytic activity of hemin is decreased due to the ion-association with Alcian blue 8GX and recovered when nucleic acids are added to the system. The change in the catalytic activity of hemin results in the fluorescence change of the hemin-catalyzed fluorogenic reaction system of p-hydroxyphenylacetic acid(p-HPA) and H2O2 and the fluorescence change of the system is proportional to the quantities of nucleic acids existing. The corresponding detect ion lim it s of the method are 0.91 ng/mL for CT DNA and 7.5 ng/mL for yeast RNA.zh_CN
dc.language.isozhzh_CN
dc.publisher中华人民共和国教育部(吉林大学承办)zh_CN
dc.subject酶催化zh_CN
dc.subject离子缔合zh_CN
dc.subject核酸zh_CN
dc.subject荧光分析zh_CN
dc.subjectEnzyme catalystzh_CN
dc.subjectIon-associationzh_CN
dc.subjectNucleic acidzh_CN
dc.subjectFluorescent analysiszh_CN
dc.title基于酶催化反应的核酸定量新方法zh_CN
dc.title.alternativeA Novel Method for the Determination of Nucleic Acids Based on Enzyme Catalyzed Fluorescence Reactionzh_CN
dc.typeArticlezh_CN


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