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dc.contributor.author郑洪zh_CN
dc.contributor.author吴敏zh_CN
dc.contributor.author李东辉zh_CN
dc.contributor.author陈秋影zh_CN
dc.contributor.author陈小兰zh_CN
dc.contributor.author许金钩zh_CN
dc.date.accessioned2011-04-26T08:04:04Z
dc.date.available2011-04-26T08:04:04Z
dc.date.issued2000-03zh_CN
dc.identifier.citation厦门大学学报(自然科学版),2000(2):191-194zh_CN
dc.identifier.issn0438-0479zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/2627
dc.description.abstract[中文文摘]以阳离子型近红外花菁染料做为生色探针 ,研究了该染料与核酸的结合反应 .在 p H6 .0的条件下 ,该染料在 771nm处有最大吸收峰 ,在核酸存在下 ,其吸收峰显著下降 ,而在 6 4 0 nm处出现新的吸收峰 .据此 ,以该近红外花菁染料为生色探针 ,在 771nm处依其吸光度的下降可定量测定核酸 .SM DNA的线性响应范围为 6 0~ 2 0 0 0 ng/ m L ,yeast RNA的线性响应范围为 10 0~ 2 0 0 0 ng/m L;检测限分别为 30 ng/ m L及 50 ng/ m L;六次平行测定 DNA(4 50 ng/ m L)的相对标准偏差为3.4 % ,测定 RNA (4 50 ng/ m L )的相对标准偏差为 3.7% ,方法具有较高的灵敏度 .用于合成试样分析, 结果满意.[英文文摘]A cationic near IR cyanine was used as a chromic probe and its binding reaction with nucleic acids was studied. In the medium of pH 6.0, the near IR cyanine showd a decrease in absorption at its maximal absorption peak (771nm) on binding to nucleic acids, and a new absorption peak appeared at 640 nm. Based on the fact, a novel near IR spectrophotometric method for the determination of nucleic acids was developed. The calibration graphs are linear over the range 60~2000 ng/mL for Salmon Sperm DNA,100~ 2 000 ng/mL for Yeast RNA , respectively. The detection lim its is 30 ng/mL for Salmon Sperm DNA , 50 ng/mL for Yeast RNA, respectively.zh_CN
dc.description.sponsorship国家自然科学基金资助项目(29775021)zh_CN
dc.language.isozhzh_CN
dc.publisher厦门大学zh_CN
dc.subject近红外花菁染料zh_CN
dc.subject核酸zh_CN
dc.subject分光光度法zh_CN
dc.subjectnear infrared spectrophotometriczh_CN
dc.subjectcyaninezh_CN
dc.subjectnucleic acidszh_CN
dc.title近红外分光光度法测定核酸zh_CN
dc.title.alternativeDetermination of Nucleic Acids by a Near-IR Spectrophotometric Methodzh_CN
dc.typeArticlezh_CN


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