Determination of Nucleic Acids Based on Shifting the Association Equilibrium between Tetrasulphonated Aluminum Phthalocyanine and Alcian Blue
- 化学化工－已发表论文 
[中文文摘]阴离子荧光染料四磺基铝酞菁与阳离子荧光染料爱尔新蓝的缔合作用 ,使四磺基铝酞菁发生荧光猝灭 ,而当核酸存在时 ,染料缔合平衡受到影响而导致四磺基铝酞菁的荧光恢复。根据这一原理 ,建立了核酸定量测定的新方法。方法具有很高的灵敏度和较好的选择性 ,其线性范围为 0～ 2 0 0 μg/L ;检测限分别为 1.8μg/L(SMDNA)、2 .0 μg/L(CTDNA)、5 .4μg/L(酵母RNA)。将方法用于实际样品金黄色葡萄球菌中DNA含量的测定 ,获得满意结果。[英文文摘]Based on the ability of nucleic acids to shift the association equilibrium of an ion association complex of alcian blue and tetra sulfonated aluminum phthalocyanine, thus leading to an increase in phthalocyanine fluorescence, a new method was suggested for the fluorimetric determination of nucleic acids. Investigations were carried out on the spectral characteristic, selection of the buffer system, effect of pH, influence of reaction time, the usage of reagents and interference of foreign substances.Under optimum conditions , the calibration curves for determinations of calf thymus deoxyribonucleic (DNA) (CTDNA) , salmon DNA (SMDNA) and yeast ribonucleic acid (RNA) were linear over the range of 2.0～200μg/ L. The detection limits for CTDNA , SMDNA and RNA were 2.0 μg/ L , 1.8μg/ L and 5.4μg/ L , respectively. This method was used to the determination of golden staplylococuus DNA and the result was in agreement with that achieved by an ultraviolet spectrophotometric method.