Selection and Identification of Differential Proteins of Liver with Proteomics Techniques in Achatina Fulica Under Induction of Cadmium
- 化学化工－已发表论文 
In comparison with direct lysis, the precipitation analysis of acetone/trichloroacetic acid was selected to optimize and separate whole proteins from the liver tissue of Achatina fulica (AF). By this method, approximately 600 protein spots in gel were obtained. Using a cabbage exposed by 0.05 mg/L CdCl2 as AF food, the liver was selected to reveal the stress proteins in the AF exposed to acute cadmium toxicity. Fourteen of differential protein spots in AF liver were obtained by proteomics, and then seven of these spots were further identified by both peptide mass fingerprint (PMF) and database search, which indicated that these proteins in part were identified to be heat shock protein, methyltransferase, adenosine triphosphate binding cassette (ABC) transporter, molybdate transporter subunit, and phosphoglycerate mutase. The results suggest that these significant proteins can be connected with metabolism pathway of cadmium, and with biomarkers for monitoring the contamination level of the cadmium and evaluating the crisis level both food and soil.
出处CHINESE JOURNAL OF ANALYTICAL CHEMISTRY，2009,37（6）：801-805