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dc.contributor.advisor任磊
dc.contributor.author张亚菲
dc.date.accessioned2016-02-14T02:13:05Z
dc.date.available2016-02-14T02:13:05Z
dc.date.issued2009-12-25 16:02:14.0
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/21983
dc.description.abstract本文旨在以硅氧烷纳米材料作为基础,引入阳离子聚合物,制备有机-无机杂化纳米基因载体。本文工作主要分为以下三个方面:1.以羧甲基壳聚糖、有机硅烷(GPSM)作为反应物,以氨水作为反应介质,在水相中制得羧甲基壳聚糖-硅氧烷(CMG)纳米颗粒。采用电子透射显微镜及粒径仪对CMG纳米颗粒的形貌、尺寸和表面电位进行表征。通过红外光谱、热失重分析等方法,对CMG纳米颗粒的结构进行了分析,并探讨了该基因载体的合成机制。为了提高该载体的功能性及结合DNA的能力,通过共价键连的方法将具有逃逸溶酶体功能的KALA活性多肽修饰到CMG纳米颗粒表面(CMG-KALA)。通过激光共聚焦、荧光光谱以及红外光谱证明KALA...
dc.description.abstractThis paper was aimed to prepare organic-inorganic hybrid nano-gene vector based on silica nano materials and cation polymers for gene delivery. The following three aspects were discussed in this paper:1. CMG nanoparticles were synthesized through a rapid one-step aqueous synthetic approach with carboxymethylchitosan, GPSM and ammonia system. The morphology, size and surface potential were characte...
dc.language.isozh_CN
dc.relation.urihttps://catalog.xmu.edu.cn/opac/openlink.php?strText=23210&doctype=ALL&strSearchType=callno
dc.source.urihttps://etd.xmu.edu.cn/detail.asp?serial=22391
dc.subject羧甲基壳聚糖-硅氧烷
dc.subject功能多肽
dc.subject体内基因转染
dc.subjectCarboxymethylchitosan-siloxane
dc.subjecttransfection in vivo
dc.subjectFunctional peptide
dc.title基于硅氧烷的基因载体的制备及体内基因转染的研究
dc.title.alternativeResearch on preparation of silica based vectors and transfection in vivo
dc.typethesis
dc.date.replied2009-06-04
dc.description.note学位:理学硕士
dc.description.note院系专业:材料学院生物材料系_生物医学工程
dc.description.note学号:24520061152678


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