甲亢和Wilson病的核磁共振代谢组学方法研究
Studies on Hyperthyroidism and Wilson’s Disease based on NMR Metabolomics
Abstract
代谢组学方法是上世纪90年代中期发展起来的一门新学科，它借助高通量、高灵敏度与高精确度的现代分析技术，分析细胞、组织和生物体液中内源性代谢物的整体组成，并通过代谢物复杂的、动态的变化，辨识和解析被研究对象的生理病理状态。本文将核磁共振代谢组学方法应用于两种代谢性疾病的研究中，甲状腺功能亢进是一种代谢性增强的疾病，Wilson病(Wilson’sdisease，WD)是机体内对铜代谢不足导致铜在体内大量积累的疾病。运用核磁共振代谢组学研究方法，分析两种代谢性疾病所导致的代谢改变，寻找了甲亢和Wilson病的特征代谢物。对甲亢疾病的研究中，进一步分析了家族性甲亢的代谢差异。对Wilson病的研究中... The quantitative measurement of the dynamic multi-parametric metabolic response of a living system to pathophysiological stimuli or genetic modification is termed “metabolomics”. The 1H NMR spectroscopy has been shown to be one of the most important analytical techniques used in metabolomics, as it can detect many endogenous metabolites rapidly and reproducibly without tendentiousness or separatio...