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dc.contributor.authorWang, Minghuazh_CN
dc.contributor.authorWang, Guizhongzh_CN
dc.contributor.author王桂忠zh_CN
dc.date.accessioned2011-04-26T08:02:35Z
dc.date.available2011-04-26T08:02:35Z
dc.date.issued2010-02zh_CN
dc.identifier.citationECOTOXICOLOGY,2010,19(2): 273-284zh_CN
dc.identifier.issn0963-9292zh_CN
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s10646-009-0410-6zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/1945
dc.description.abstractTigriopus japonicus Mori has been recognized as a good model for toxicological testing of marine pollutants. Recently, a large number of genes have been identified from this copepod, and their mRNA expression has been studied independently against exposure to marine pollutants; however, biochemical-response information is relatively scarce. The response of T. japonicus to nickel (Ni) additions was examined under laboratory-controlled conditions in 12 days exposure. Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), acetylcholinesterase (AchE), reduced glutathione (GSH), the ratio of reduced to oxidized glutathione (GSH/GSSG) and metallothionein (MT) were analyzed for Ni treatments (0, 0.125, 0.25, 0.75 and 3.0 mg/L) after 1, 4, 7 and 12 days. The thiobarbituric reactive species assay was used to evaluate lipid peroxidation (LPO) level in copepods after exposure. The results showed that Ni remarkably affected the biochemical parameters (SOD, GPx, GST, GSH, and GSH/GSSG) after certain exposure durations. However, the copepod's LPO level was significantly decreased under metal treatments after exposure, hinting that the factors involved in LPO might not significantly depend on the operations and functions in the antioxidant system. Ni exhibited the neurotoxicity to copepods, because its use obviously elevated AchE activity. During exposure, Ni initially displayed an inhibition effect but induced MT synthesis in T. japonicus by day 12, probably being responsible for metal detoxification. Thus, Ni had intervened in the detoxification process and antioxidant system of this copepod, and it could be used as a suitable bioindicator of Ni exposure via measuring SOD, GPx, GST, and MT as biomarkers.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [40876060]zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGERzh_CN
dc.subjectBiomarkerzh_CN
dc.subjectCopepodzh_CN
dc.subjectMetallothioneinzh_CN
dc.subjectNickelzh_CN
dc.subjectTigriopus japonicuszh_CN
dc.titleOxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickelzh_CN
dc.typeArticlezh_CN


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