海水养殖沉积环境细菌多样性PCR-DGGE分析

Abstract

[中文文摘]采用PCR结合变形梯度凝胶电泳(PCR-DGGE)技术,分析泉州东海海水养殖池基地新建虾池与一15年池龄虾池沉积物样品中细菌群落组成的变化.对DGGE图谱中的13条主要条带进行测序分析,结果表明,虾池环境中的主要微生物类群为γ-变形杆菌亚群(γ-proteobacteria,30.77%)、厚壁菌群(Firmicutes,15.38%)、δ-变形杆菌亚群(δ-proteobacteria,7.6%)、拟杆菌群(Bacteroidetes,7.6%)、绿弯菌群(Chloroflexi,7.6%),放线菌群(Actinobacteria,7.6%),未知菌群(Unknown environmental samples)占23.08%. [英文文摘]Bio div ersity of bacter ia in the sediment fr om a new and an o ld shrimp and crab mix ed- cultur ed po nd is studied by a combinat ion of PCR and DGGE. Phy lo genetic analysis show s that bacter ial community compo sitio n o f the sediment has a high diver sity. T he clo nes from DGGE pr ofile fall into seven bacteria g roups, including C-p roteobacter ia( 30. 77%) , Firmicutes (15. 38%) , D-p r oteobacter ia( 7. 6%) , B acter oidetes( 7. 6%) , Chlo rof lex i( 7. 6%) , A ctinobacter ia( 7. 6%) . In addition, a par t of Unknow n env iro nmental samples( 23. 08%) is detected.