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dc.contributor.authorYe, Haihuizh_CN
dc.contributor.author叶海辉zh_CN
dc.contributor.authorSong, Pingzh_CN
dc.contributor.author宋平zh_CN
dc.contributor.authorHuang, Huiyangzh_CN
dc.contributor.author黄辉洋zh_CN
dc.contributor.authorWang, Guizhongzh_CN
dc.contributor.author王桂忠zh_CN
dc.contributor.authorMa, Jun(Univ Penn, Dept Biol, Philadelphia, PA 19104USA)zh_CN
dc.date.accessioned2011-04-26T08:02:32Z
dc.date.available2011-04-26T08:02:32Z
dc.date.issued2010-01zh_CN
dc.identifier.citationMARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY,2010,43(1):25-35zh_CN
dc.identifier.issn1023-6244zh_CN
dc.identifier.urihttp://dx.doi.org/doi:10.1080/10236241003654113zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/1907
dc.description.abstractVertebrate-type steroids, such as progesterone, have been identified in crustaceans. The physiological activity of progesterone during vitellogenesis is still not well understood. In this study, progesterone levels in the female mud crab, Scylla paramamosain, were determined by enzyme-linked immunosorbent assay. Peak levels of progesterone were detected during the previtellogenic stage in the hemolymph, ovary, and hepatopancreas, whereas the progesterone level decreased significantly in vitellogenic stage I. During vitellogenic stage II, progesterone levels rose again in the hemolymph and ovary, but continued to decrease in the hepatopancreas. By using western blotting, progesterone receptor (PR), with an apparent molecular weight of 70 kDa, was identified in the ovary during both vitellogenic stages I and II. By means of immunohistochemistry, PR was detected mainly in the follicle cells during vitellogenic stage I and in the nuclei of oocytes in vitellogenic stage II. Our results strongly suggest that progesterone promotes vitellogenesis in the mud crab, S. paramamosain via a classical genomic mechanism.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [40406030, 40776084]; National High Technology Research, and Development Program of China [2006AA10A406]zh_CN
dc.language.isoenzh_CN
dc.publisherTAYLOR & FRANCIS LTDzh_CN
dc.subjectcrabzh_CN
dc.subjecthormoneszh_CN
dc.subjectprogesteronezh_CN
dc.subjectprogesterone receptorzh_CN
dc.subjectvitellogenesiszh_CN
dc.subjectScylla paramamosainzh_CN
dc.titleChanges in progesterone levels and distribution of progesterone receptor during vitellogenesis in the female mud crab (Scylla paramamosain)zh_CN
dc.typeArticlezh_CN


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