Show simple item record

dc.contributor.author张玉红
dc.contributor.author于金金
dc.contributor.author吴晓琛
dc.contributor.author田惠桥
dc.date.accessioned2013-05-22T01:22:51Z
dc.date.available2013-05-22T01:22:51Z
dc.date.issued2013-01
dc.identifier.citation亚热带植物科学,2013,42(1):5-9zh_CN
dc.identifier.issn1009-7791
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/18686
dc.description作者简介:张玉红(1988-),女,山东乳山人,硕士研究生,从事植物学研究。E-mail: 1072970432@qq.com 注:田惠桥为通讯作者。E-mail: hqtian@xmu.edu.cnzh_CN
dc.description.abstract杜鹃成熟花粉为二胞型,含一个营养细胞和一个生殖细胞,其精细胞在花粉管中形成。应用半离体技术培养杜鹃已授粉花柱,使花粉管从花柱中长出,再用渗透压冲击法促使花粉管破裂,释放出一对与营养核相连的精细胞。分离的精细胞经FDA方法检测,证明具活性。用显微操作仪可收集数量较多的分离精细胞。[英文文摘]The mature pollen of Rhododendron simsii is bicellular, containing one vegetative cell and one germ cell, with the sperm cells forming in pollen tube. Semi vitro technique was used in style pollinated to make pollen tube sprout from style. Then the pollen tube burst by osmotic shock method and released a pair of sperm cells which connected to vegetative nucleus. The sperm cells separated kept activities proved by FDA method. A fairly large number of separated sperm cells could be collected by micromanipulator.zh_CN
dc.description.sponsorship国家自然科学基金(30970275、31170289);公益性行业(农业)科研专项课题(200903016)资助zh_CN
dc.language.isozhzh_CN
dc.publisher《亚热带植物科学》编辑部zh_CN
dc.subject杜鹃zh_CN
dc.subject精细胞zh_CN
dc.subject分离zh_CN
dc.title杜鹃精细胞的分离zh_CN
dc.title.alternativeSeparation of Sperm Cells of Rhododendron simsiizh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record