β-catenin拮抗因子Chibby在鼻咽癌中的表达及意义

Abstract

[中文文摘]目的研究β-catenin拮抗因子Chibby在鼻咽癌组织中的表达情况,并探讨其与鼻咽癌临床病理因素的相关性。方法分别采用Real time PCR及免疫组织化学(SABC法)检测45例鼻咽癌及癌旁正常鼻咽黏膜组织中Chibby mRNA及蛋白的表达情况,统计Chibby的表达与鼻咽癌患者临床病理因素的关系。结果 Chibby mRNA在癌旁正常鼻咽黏膜及鼻咽癌组织中平均相对表达量分别为0.0835±0.0056和0.0776±0.0059,Chibby蛋白在癌旁正常鼻咽黏膜及鼻咽癌组织中的阳性表达率分别为91.1%(41/45)和42.2%(19/45)。鼻咽癌组织中Chibby的表达与T分期及临床分期有相关性,与性别、年龄、颈淋巴结转移及病理分型无明显相关性。结论与癌旁正常鼻咽黏膜组织相比,鼻咽癌组织中Chibby mRNA及蛋白的表达均降低,Chibby的表达与肿瘤T分期及临床分期相关,与性别、年龄、颈淋巴结转移及病理分型无明显相关性,Chibby可能参与了鼻咽癌的发生过程,有望成为鼻咽癌基因治疗新的作用靶点。[英文文摘]OBJ ECTIVE TO investigate the expression of Chibby a new β-catenin inhibitor in nasopharyngeal careinoma , and to explore the relationship between Chibby and clinicopathological features of nasopharyngeal carcinoma . METHODS Real time PCR and SABC im munohistochemical staining were used to detect the expression of Chibby mR N A and protein in nasopharyngeal carcinoma tissues and nasopharyngeal normal tissues of 45 cases , respectively .The re!ationships between Chibby expression and cIinicopathological features the gender, age , T stages .Iymph node metastasis , clinical stages and pathological grade were analyzed by statistics . RULTS The expressions of Chibby mRNA were 0 .0835士0 .0056 and0.0776 士0.0059 in nasopharyngeal normal tissues and nasopharyngeal carcinoma tissues, respectively.The positive expressive rates of chibby protein were 91.1 % (41/45 ) and 42 .2 % (19/45 ) in nasopharyngeal norma l tissues and nasopharyngeal carcinoma tissues,respectively.There were eminent relation between the lower expression of Chibby in nasopharyngeal careinoma and T stages and clinical stges , but no relation with the sex , age , metastasis of lymph and pathological grades .CONCLUSION Compare with normal nasopharyngea l tissues, the expressions of Chibby mRNA and prote in were reduced in nasopharyngeal carcinoma tissues.The Iower expressive levels of Chibby were associated with the T stages and clinica l stages of carcinoma , but no relation with the sex , age , metastasis of Iymph and pathological grades.Chibby may be involved in the pathogenesis of nasopharyngeal carcinoma ,and be expected to become a new target of gene t herapy of it .