Influence of LDAO on the Conformation and Release of Bacteriochlorophyll of Peripheral Light-Harvesting Complex (LH2) from Rhodobacter Azotoformans
Zhao, Gen-gui ( Huaqiao Univ, Dept Bioengn & Biotechnol, Xiamen 361021, Peoples R China )
Dong, Yan-min ( Shanxi Univ, Key Lab Chem Biol & Mol Engn, Minist Educ, Sch Life Sci & Technol, Taiyuan 030006, Peoples R China)
Yang, Su-ping（Huaqiao Univ, Dept Bioengn & Biotechnol, Xiamen 361021, Peoples R China）
Qu, Yin-bo（Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China）
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The aim of this study is to reveal the interaction relationships between lauryl dimethylamine N-oxide (LDAO) and peripheral light-harvesting complex (LH2) as well as the influence of LDAO on structure and function of LH2. In the present work, the effects of LDAO on the conformation and release processes of bacteriochlorophyll (BChl) of LH2 when incubated under different temperature and pH in the presence and absence of LDAO were investigated by spectroscopy. The results indicated that (1) the presence of LDAO resulted in alterations in the conformation, a-helix content, and spectra of Tyr and B850 band of LH2 at room temperature and pH 8. 0. Moreover, energy transfer efficiency of LH2 was enhanced markedly in the presence of LDAO. (2) At 60 degrees C, both the 13800 and 13850 band of LH2 were released and transited into free BChl at pH 8. 0. However, the release rates of bacteriochlorophylls of B800 and 13850 band from LH2 were slowed clown and the release processes were changed when incubated in the presence of LDAO. Hence, the stability of LH2 was improved in the presence of LDAO. (3) The accelerated release processes of bacteriochlorophylls of B800 and 13850 band of LH2 were induced to transform into bacteriopheophytin (BPhe) and free BChl by LDAO in strong acid and strong alkalic solution at room temperature. However, the kinetic patterns of the B800 and B850 band were remarkably different. The release and self-assemble processes of B850 in LH2 were observed in strong acid solution without LDAO. Therefore, the different release behaviors of 13800 and 13850 of LH2 are induced by LDAO under different extreme environmental conditions.
CitationSPECTROSCOPY AND SPECTRAL ANALYSIS ,2010,30（10）： 2807-2811