金定鸭遗传多样性及分子标记的研究
Studies on Genetic Diversity and Molecular Marker of Jinding Duck
Abstract
[中文文摘]用40个随机引物对我国常见家鸭品种(系)、引进品种和野鸭基因组DNA进行RAPD分析,寻找金定鸭品种特征RAPD标记.通过多次重复实验,初步认为Y05 600可作为鉴别育成品种金定鸭的RAPD分子标记.再选用20个引物对金定鸭3个育成品系各12个个体进行RAPD扩增,分析金定鸭的种质特征.结果显示:金定鸭I、II、III系的平均遗传相似系数分别为0.9157±0.0296、0.8552±0.0459、0.8816±0.0449;3个品系的变异系数分别为3.23%、5.37%、5.09%.育成品种金定鸭不同品系遗传一致性较高,但品系内的个体间仍存在着丰富的遗传多样性,不同品系之间存在着极显著的差异(p<0.01).[英文文摘]In order to find the molecular markers of Jinding Duck, the random amplified polymorphic DNA (RAPD) was used to analyze the genomic DNA of 11 strains of domestic ducks and two species of wild duck. The result showed that the band Y_(05-600) was a particular band in all Jinding duck samples. It demonstrated that the band maybe a RAPD marker, which could distinguish Jinding breed from other ducks. Twenty primers were chosen for analysing the genetic diversity in 36 individuals belonging to three Jinding ducklines. The result showed that the average genetic similar coef ficient among them was 0. 915 7 ± 0. 029 6, 0. 855 2 ±0. 045 9 and 0. 881 6 ± 0. 044 9. The coeff icient variability of three lines was 3. 23% , 5. 37% and 5. 09% , respect ively.The variance among the three lines was very significant ( P< 0. 01) .