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dc.contributor.authorLiu Jumei
dc.contributor.authorZhang Ye
dc.contributor.authorXie Huabin
dc.contributor.authorZhao Li
dc.contributor.authorZheng Lei
dc.contributor.authorYe Huiming
dc.date.accessioned2020-10-10T02:09:23Z
dc.date.available2020-10-10T02:09:23Z
dc.date.issued2019-10-28
dc.identifier.citationSmall (Weinheim an der Bergstrasse, Germany),2019,(42)
dc.identifier.other10.1002/smll.201902989
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/174863
dc.description.abstractNucleic acids are considered as perfect programmable materials for cascade signal amplification and not merely as genetic information carriers. Among them, catalytic hairpin assembly (CHA), an enzyme-free, high-efficiency, and isothermal amplification method, is a typical example. A typical CHA reaction is initiated by single-stranded analytes, and substrate hairpins are successively opened, resulting in thermodynamically stable duplexes. CHA circuits, which were first proposed in 2008, present dozens of systems today. Through in-depth research on mechanisms, the CHA circuits have been continuously enriched with diverse reaction systems and improved analytical performance. After a short time, the CHA reaction can realize exponential amplification under isothermal conditions. Under certain conditions, the CHA reaction can even achieve 600 000-fold signal amplification. Owing to its promising versatility, CHA is able to be applied for analysis of various markers in vitro and in living cells. Also, CHA is integrated with nanomaterials and other molecular biotechnologies to produce diverse readouts. Herein, the varied CHA mechanisms, hairpin designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA are presented. Finally, challenges and the outlook of CHA development are considered.
dc.language.isozh_CN
dc.subjectBiosensors
dc.subjectCatalytic hairpin assembly (CHA)
dc.subjectIsothermal amplification
dc.subjectNucleic acid circuits
dc.subjectToehold mediated strand displacement
dc.titleApplications of Catalytic Hairpin Assembly Reaction in Biosensing.
dc.typeArticle


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