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dc.contributor.authorGao Z-X
dc.contributor.authorLuo X
dc.contributor.authorLiu L-L
dc.contributor.authorLin L-R
dc.contributor.authorTong M-L
dc.contributor.authorYang T-C
dc.date.accessioned2020-10-10T02:09:23Z
dc.date.available2020-10-10T02:09:23Z
dc.date.issued2019-10-28
dc.identifier.citationJournal of the European Academy of Dermatology and Venereology : JEADV,2019,(10)
dc.identifier.other10.1111/jdv.15725
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/174862
dc.description.abstractBACKGROUND(#br)Although angiogenesis is an obvious pathological manifestation in the pathogenesis of syphilis, little is known about the underlying mechanisms of angiogenesis induced by reactions to Treponema pallidum antigens.(#br)OBJECTIVE(#br)In this study, we sought to determine the role of recombinant T. pallidum Tp47 in promoting angiogenesis in endothelial cells and the related mechanism.(#br)METHODS(#br)Evaluation of the pro-angiogenic activity of recombinant T. pallidum Tp47 in human umbilical vein endothelial cells (HUVECs) was assessed, and the balance of matrix metalloproteinase (MMP)/tissue inhibitor of metalloproteinase (TIMP) and the mechanisms underlying the involvement of Akt/mTOR/S6 pathways in this process were explored.(#br)RESULTS(#br)Under stimulation by Tp47, HUVECs exhibited obvious proliferation, migration and tube formation. In addition, the apparent promotion of angiogenesis by Tp47 was observed using a zebrafish embryo model. During angiogenesis, the levels of MMP-1 and MMP-10 were significantly elevated, whereas those of TIMP-1 and TIMP-2 did not change. In addition, after transfection with siRNAMMP-1 and siRNAMMP-10, migration and tube formation were significantly inhibited. Akt/mTOR/S6 signalling was found to be involved in upregulating MMP-1 and MMP-10 expression, and the sequential blockade of steps in the pathways effectively prevented Tp47-induced angiogenesis.(#br)CONCLUSION(#br)The results reveal the underlying mechanism of angiogenesis promoted by Tp47, namely, upregulating MMP-1 and MMP-10 expression to disrupt the MMP/TIMP balance through the Akt/mTOR/S6 pathway. These findings contribute to our understanding of the pathophysiology of syphilis.
dc.language.isozh_CN
dc.titleRecombinant Treponema pallidum protein Tp47 induces angiogenesis by modulating the matrix metalloproteinase/tissue inhibitor of metalloproteinase balance in endothelial cells.
dc.typeArticle


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