SNX27调节突触传导和突触可塑性

Abstract

摘要 目的：SNX27作为一种重要的分选蛋白，主要参与膜受体从内涵体重新转运到细胞膜上的过程，SNX27的缺失会导致到突触受体循环过程受到阻碍。本研究通过构建人SNX27转基因小鼠探讨过表达SNX27对于突触功能的影响。 方法：采用SNX27转基因小鼠作为研究对象，通过Camk2a启动子诱导人SNX27基因在小鼠皮层以及海马中过表达。在一月龄SNX27转基因小鼠和WT小鼠海马脑片上检测CA1区锥体神经元（Pyramidalneuron）的微小兴奋性/抑制性突触后电流（miniatureexcitatory/inhibitorypostsynapticcurrents，mEPSCs/mIPS...

Abstract Objective：Among sorting nexin family proteins, SNX27 is a unique one as it plays crucial role in sorting and recycling transmembrane proteins from endosome-to-plasma membrane. SNX27 deficiency disrupts trafficking of synaptic receptors and leads to synaptic dysfunction. In this study, we will determine the functions of SNX27 using aSNX27 transgenic mouse model. Method：In order to stud...