样本处理流程对血液中梅毒螺旋体DNA提取效率的影响
Assessing the effects of different processing procedures on the yield of Treponema pallidum DNA from blood
Abstract
背景:聚合酶链反应（Polymerasechainreaction，PCR）可直接检测梅毒螺旋体（Treponemapallidumsubsp.pallidum，T.pallidum），对梅毒进行明确诊断。然而梅毒患者血液样本的T.pallidum-PCR阳性率低，常与临床诊断不符。本研究探讨样本处理流程和血液样本类型对T.pallidumDNA得率的影响，为提高血液T.pallidum-PCR的阳性率提供实验依据。 方法:采用自主构建的Tp47基因荧光定量PCR方法检测一系列体外模拟实验样本和感染兔血中的T.pallidumDNA，探讨不同样本处理流程对T.pallidumDNA提取效率的... Object: Polymerase chain reaction (PCR) is a method to detect the Treponema pallidum subsp. pallidum (T. pallidum) and provides definitive diagnosis of syphilis. However, the sensitivity of T. pallidum blood-PCR is moderate, giving a low level of concordance with the physician’s diagnosis. In this study, we explore a study on the effects of different clinical sample processing procedures and diffe...